氯吡格雷对斑马鱼生物钟及生物节律的影响

发布时间：2018-06-17 23:21

本文选题：氯吡格雷 + 斑马鱼　；参考：《中国医院药学杂志》2017年14期

【摘要】：目的:研究不同剂量氯吡格雷对斑马鱼体大脑核心钟基因bmal,clock,cry和per表达及其生物节律的影响。方法:采用不同浓度氯吡格雷处理斑马鱼1 h后,收集并观察脑组织中生物钟基因bmal、clock、cry、per的mRNA转录水平,并观察氯吡格雷对斑马鱼生物节律,如活动振幅、时相、行为周期和体内褪黑素水平的影响。结果:通过对氯吡格雷处理后斑马鱼大脑中生物钟基因bmal、clock、cry、per的转录水平进行分析发现,氯吡格雷可以显著下调bmal1a、bmal2、clock1a、cry1aa、per1a及per2的表达(P0.05),同时clock2、cry1bb、per1b及per3的转录水平与氯吡格雷成正相关,发挥应激性补偿作用。经过氯吡格雷处理,斑马鱼的活动振幅和时相显著下调(P0.05),同时体内褪黑素水平极显著降低(P0.001)。结论:氯吡格雷摄入导致斑马鱼大脑中主要生物钟基因表达紊乱,影响正常的生物节律及调节睡眠关键激素-褪黑素的分泌水平。
[Abstract]:Aim: to study the effects of different doses of clopidogrel on the expression and biological rhythm of bmalloclockcry and per in zebrafish. Methods: zebrafish were treated with clopidogrel at different concentrations for 1 h. The mRNA transcription level of the clock gene bmalloclockcryper in brain tissue was collected and observed. The biological rhythm of zebrafish, such as the amplitude of activity and the temporal phase, was observed by clopidogrel, and the effects of clopidogrel on the biological rhythm of zebrafish were studied. Effects of behavioral cycle and melatonin levels in vivo. Results: after treatment with clopidogrel, we found that clopidogrel could significantly down-regulate the expression of bmal1ahbbper1a and per2, and the transcription levels of clock 2cry1bbper1b and per3 were positively correlated with clopidogrel. Play the role of stress compensation. After treatment with clopidogrel, the activity amplitude and time phase of zebrafish decreased significantly (P 0.05), while the level of melatonin in vivo decreased significantly (P 0.001). Conclusion: clopidogrel intake leads to the disturbance of the expression of the main biological clock gene in zebrafish brain, which affects the normal biological rhythm and regulates the secretion of melatonin, the key hormone in sleep.
【作者单位】：齐齐哈尔医学院附属第二医院;哈尔滨医科大学附属第二医院;
【基金】：齐齐哈尔市科学计划项目(编号:SFZD2014041)
【分类号】：R965

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