HPLC法测定氯霉素滴眼液在兔眼房水中的质量浓度

发布时间：2018-06-19 22:48

本文选题：高效液相色谱法 + 氯霉素滴眼液　；参考：《中国药房》2015年07期

【摘要】：目的:测定氯霉素滴眼液在兔眼房水中的质量浓度。方法:在6只兔眼结膜囊内滴入氯霉素滴眼液100μl,于滴眼后5、15、30、60、120、180 min取眼房水,加甲醇后涡旋、离心,高效液相色谱法测定上清液中氯霉素质量浓度。色谱柱为Hypersil ODS C18,流动相为甲醇-0.34%磷酸二氢钾水溶液(55∶45),检测波长为273 nm,流速为1.0 ml/min,柱温为30℃,进样量为20μl。结果:氯霉素检测质量浓度的线性范围为0.125~4μg/ml(r=0.998 6,n=6),方法回收率为91.28%~98.35%、提取回收率为88.89%~96.92%(n=3),日内RSD为2.98%、日间RSD为3.34%(n=5);各时间点眼房水样品中氯霉素质量浓度分别为0、(0.331±0.041)、(0.251±0.049)、(0.133±0.052)、(0.068±0.042)、(0.035±0.043)μg/ml。结论:本方法灵敏、结果准确,可用于兔眼房水中氯霉素质量浓度的测定。
[Abstract]:Objective: to determine the mass concentration of Chloramphenicol Eye Drops in the aqueous humor of rabbit eyes. Methods: in the conjunctival sac of 6 rabbit eyes, Chloramphenicol Eye Drops 100 L was dripped into the conjunctival sac, the aqueous humor of the eye was taken by 5,15,30,60120180 min after the eye drops. The mass concentration of chloromycin in the supernatant was determined by high performance liquid chromatography. The chromatographic column was Hypersil ODS C18. The phase is methanol -0.34% phosphate dihydrogen phosphate solution (55: 45), the detection wavelength is 273 nm, the flow rate is 1 ml/min, the column temperature is 30, and the sample volume is 20 L.. The linear range of the quality concentration of chloramphenicol is 0.125~4 mu g/ml (r=0.998 6, n=6), the recovery rate is 91.28%~ 98.35%, the recovery rate is 88.89%~96.92% (n=3), and the day RSD is 2.98%, day 2.98%, day 2.98%. The RSD was 3.34% (n=5), and the concentration of chloramphenicol in water samples at each time was 0, (0.331 + 0.041), (0.251 + 0.049), (0.133 + 0.052), (0.068 + 0.042) and (0.035 + 0.043) micron g/ml. conclusion: this method is sensitive and accurate, and can be used for the determination of chloramphenicol in aqueous humor of rabbit eyes.
【作者单位】：重庆市渝北区人民医院;
【分类号】：R927.2;O657.72

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