叔丁基过氧化氢体外诱导人肾小球系膜细胞的衰老

发布时间：2018-06-20 00:21

本文选题：叔丁基过氧化氢(t-BHp) + 肾小球系膜细胞　；参考：《中国老年学杂志》2015年01期

【摘要】：目的通过观察不同浓度叔丁基过氧化氢(t-BHP)作用不同时间后对人肾小球系膜细胞(HGMC)衰老指标的影响,探讨t-BHP在HGMC衰老过程中的作用。方法应用10、30、50、100μmol/L t BHP刺激细胞,每天刺激1 h,连续5 d,刺激结束后48 h检测细胞增殖情况、衰老相关β半乳糖苷酶(SAβ-gal)染色阳性细胞百分率、细胞周期变化及细胞形态改变确定t-BHP诱导HGMC衰老的最佳浓度。结果 30μmol/L t BHP刺激HGMC,每天1 h连续作用5次后可抑制HGMC增殖,且84%的HGMC SAβ-gal染色阳性,90%HGMC进入G0~G1期;光镜表现细胞体积增大、胞体扁平、胞质颗粒增多;电镜下细胞核膜内陷,染色质凝集、边集,细胞质空泡增多,出现髓样溶酶体。结论 30μmol/L t BHP刺激HGMC后每天1 h连续作用5次,可作为诱导HGMC发生衰老的刺激因素。
[Abstract]:Objective to investigate the role of t-BHP in the aging of human glomerular Mesangial cells (HGMC) by observing the effects of different concentrations of t-BHPH on the aging of human glomerular Mesangial cells (HGMCs). Methods the cells were stimulated with 1030g / L 50 渭 mol / L t BHP for 1 hour for 5 days. After 48 hours of stimulation, the proliferation of cells and the percentage of positive cells with 尾 -galactosidase SA 尾 -galactosidase staining were detected. Cell cycle changes and cell morphology changes determined the optimal concentration of t-BHP induced HGMC senescence. Results HGMC stimulated by 30 渭 mol / L t BHP could inhibit the proliferation of HGMC after continuous treatment for 1 hour and 5 times a day, and 84% of HGMC SA 尾 -gal positive cells entered G0 / G 1 phase. Light microscopy showed that the cell volume was larger, the cell body was flat and the cytoplasmic granules were increased, and the nuclear membrane of HGMC was trapped under electron microscope. Chromatin agglutination, edge agglutination, cytoplasmic vacuoles increased and myeloid lysosomes appeared. Conclusion 30 渭 mol / L t BHP can be used as a stimulant to induce HGMC to senescence.
【作者单位】：辽宁医学院附属第一医院;
【基金】：辽宁省自然科学基金项目(No.2013022038) 辽宁医学院校长基金奥鸿博泽基金(No.XZJJ20130249)
【分类号】：R96

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