离子色谱快速检测壳寡糖含量

发布时间：2018-06-21 13:40

  本文选题：壳聚糖 + 壳寡糖　； 参考：《药物分析杂志》2017年11期

【摘要】：目的:为了快速准确的进行壳寡糖样品的检测,建立一种分离度好、灵敏度高的壳寡糖离子色谱快速检测方法。方法:实验选用Dionex-Carbo Pac PA104×50 mm离子交换色谱柱,通过优化柱温、淋洗液配比及浓度等色谱条件,将不同聚合度的壳寡糖在30 min内实现分离。分离后的壳寡糖样品通过计算聚合度为壳2~6糖的含量,检验其样品中壳寡糖的纯度。结果:样品浓度在2.00~10.00μg·m L~(-1)范围内线性关系良好(r≥0.999 0),精密度实验RSD在0.1%~1.6%之间,样品加标回收率93.2%~105.0%。3种壳寡糖样品中2~6个聚合度壳寡糖的含量之和分别为25.944μg·g~(-1)、26.406μg·g~(-1)、25.374μg·g~(-1),计算得到纯度为86.5%、88.0%和84.6%,符合国家标准要求。结论:该方法需要样品量少,检验快速,无需衍生,为壳寡糖质量分析、组分分离提供了依据。
[Abstract]:Objective: to establish a rapid and sensitive method for the determination of chitosan oligosaccharides by ion chromatography. Methods: Dionex-Carbo PA104 脳 50mm ion exchange column was used to separate chitosan oligosaccharides with different degree of polymerization within 30 min by optimizing the column temperature, eluant ratio and concentration. The purity of chitosan oligosaccharide was tested by calculating the degree of polymerization of chitosan oligosaccharide to be the content of chitosan oligosaccharide. Results: the linear range of sample concentration was 2.00 渭 g / L ~ (-1), r 鈮，

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