杏鲍菇多糖对S180荷瘤小鼠的保护作用

发布时间：2018-06-22 15:13

本文选题：杏鲍菇多糖 + S肿瘤细胞　；参考：《食品科学》2015年03期

【摘要】：目的:分析杏鲍菇多糖对S180荷瘤小鼠的保护能力和对化疗效果的影响。方法:将小鼠随机分为6 组:空白对照组(A组)、用糖对照组(B组)、肿瘤模型组(C组)、化疗组(D组)、感染用糖组(E组)和化疗用糖组(F组)。其中"用糖"是指在40 d的实验期间内一直腹腔注射100 mg/(kg·d)(以体质量计,下同)杏鲍菇多糖,"感染"是指在实验第1天一次性后肢皮下注射3 mL/kg新鲜制备的含有S180肿瘤细胞的种鼠腹水;"化疗"是指小鼠接种S180细胞10 d后腹腔注射5.0 mg/kg顺铂,间隔1 d注射一次,共注射5 次。接种20～40 d时观测死亡率、体质量、白细胞数量和腹水超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性及丙二醛(malondialdehyde,MDA)浓度。结果:杏鲍菇多糖能够显著降低S180荷瘤小鼠的死亡率,延长小鼠存活时间,增加血液内白细胞数量,增强腹水SOD和CAT的酶活性,降低MDA浓度,降低化疗对小鼠免疫能力的影响(P0.05)。结论:杏鲍菇多糖对S180荷瘤小鼠具有极强的保护作用,并且能够降低顺铂化疗的副作用。
[Abstract]:Objective: to analyze the protective effect of Pleurotus eryngii polysaccharides on S 180 tumor bearing mice and its effect on chemotherapy. Methods: the mice were randomly divided into six groups: blank control group (group A), sugar control group (group B), tumor model group (group C), chemotherapy group (group D), infected group (group E) and chemotherapy group (group F). "Sugar" refers to the use of 100 mg/ (kg d) (intraperitoneal injection for a period of 40 days to measure the body mass. Eryngium eryngii polysaccharides "infection" refers to the ascites of mice containing S180 tumor cells, which were prepared by subcutaneous injection of 3 mL / kg on the first day of the experiment. "chemotherapy" refers to the mice inoculated with S180 cells for 10 days after intraperitoneal injection of 5.0 mg/kg cisplatin. Once a day, a total of 5 injections. Mortality, body mass, white blood cell count, superoxide dismutase (superoxide dismutase), catalase (cat) activity and malondialdehyde (malondialdehyde) concentration were measured at 20 ~ 40 d after inoculation. Results: Pleurotus eryngii polysaccharides could significantly reduce the mortality, prolong the survival time, increase the number of white blood cells, enhance the activities of SOD and cat in ascites, decrease the concentration of MDA and decrease the effect of chemotherapy on the immune ability of S180 mice (P0.05). Conclusion: Pleurotus eryngii polysaccharide has a strong protective effect on S 180 tumor bearing mice and can reduce the side effects of cisplatin chemotherapy.
【作者单位】：唐山市农业科学研究院;天津市宝坻区第四中学;
【基金】：河北省自然科学基金项目(10220126)
【分类号】：R965

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