HPLC法测定伊潘立酮的含量及有关物质

发布时间：2018-06-24 04:28

本文选题：伊潘立酮 + 含量　；参考：《中国药师》2016年12期

【摘要】：目的:建立伊潘立酮的含量及有关物质测定方法。方法:采用HPLC法,色谱柱:VP-ODS C18柱(150 mm×4.6 mm,5μm);流动相:甲醇-0.1 mol·L~(-1)磷酸氢二铵溶液(用磷酸调pH至6.2±0.1)(65∶35);流速:1.0 ml·min~(-1);检测波长:275nm和228 nm;柱温:30℃;进样量:20μl。结果:本方法能较好地分离伊潘立酮及其有关物质,伊潘立酮在10.08~100.80μg·ml~(-1)浓度范围内线性关系良好(r=0.999 8),平均回收率为100.2%(RSD=0.82%,n=9)。结论:该方法灵敏快速、结果准确、重复性好,可用于伊潘立酮原料药的含量测定及有关物质检查。
[Abstract]:Objective: to establish a method for the determination of the content and related substances of peridone. Methods: using HPLC method, chromatographic column: VP-ODS C18 column (150 mm x 4.6 mm, 5 micron m); mobile phase: methanol -0.1 mol. L~ (-1) dihydrogen phosphate solution (pH to 6.2 + 0.1) (65: 35); flow velocity: 1 ml. 228; column temperature: 30 C; sampling amount: 20 micron Results: the method was good for isolating peridone and its related substances. The linear relationship of peridone in the concentration range of 10.08~100.80 mu g. Ml~ (-1) was good (r=0.999 8). The average recovery rate was 100.2% (RSD=0.82%, n=9). Conclusion: the method is sensitive, rapid, accurate and reproducible. It can be used for the determination of the content of apperrisone and the related content of the drug. Physical examination.
【作者单位】：武汉大学中南医院药学部;
【分类号】：R927.2;O657.72

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