载柚皮素壳聚糖纳米粒的制备及其体外细胞评价

发布时间：2018-06-24 16:16

本文选题：柚皮素 + 壳聚糖纳米粒　；参考：《中国医院药学杂志》2017年20期

【摘要】：目的:制备柚皮素壳聚糖纳米粒,初步探讨其对人肺腺癌细胞A549的细胞毒性和细胞摄取。方法:以壳聚糖和鱼精蛋白作为载体材料,采用离子胶凝法制备柚皮素壳聚糖纳米粒,透射电镜(TEM)观察其形态,马尔文激光粒度仪测定其粒径、分散度(PDI)和Zeta电位,离心法测定其包封率和载药量,采用恒温振荡水浴法对柚皮素壳聚糖纳米粒进行体外释放度研究,最后采用人肺癌细胞系A549细胞进行了细胞毒性、细胞摄取研究。结果:柚皮素壳聚糖纳米粒为球形或类球形粒子,结构完整,大小均一、球形度好,分散均匀,PDI、粒径、Zeta电位和包封率分别为0.268,139 nm、+15.7 mV和83.34%,柚皮素壳聚糖纳米粒体外释放呈缓释,24 h累积释放量达到了80%以上,体外释药过程用Higuchi方程拟合较好。MTT试验显示不同浓度的壳聚糖纳米粒和细胞作用72 h后,细胞活力均大于95%,本文所制备的壳聚糖纳米粒无细胞毒性。细胞摄取试验表明载FITC的壳聚糖纳米粒和A549细胞作用3 h后,可明显看到大量带绿色荧光的纳米粒穿过细胞膜进入细胞。结论:离子凝胶法成功制得粒径较小的柚皮素壳聚糖纳米粒,具有缓释性好,毒性小,壳聚糖纳米粒摄取率较高,可大大提高药物的利用率,具有广泛的应用前景。
[Abstract]:Objective: to prepare naringin chitosan nanoparticles and to investigate the cytotoxicity and cell uptake of human lung adenocarcinoma cell A549. Methods: Chitosan and protamine were used as carrier materials, and naringin chitosan nanoparticles were prepared by ion cementitious method, and the morphology of TEM was observed by transmission electron microscopy (TEM). The particle size and dispersion of the chitosan nanoparticles were measured by Malvin laser particle size analyzer. Degree (PDI) and Zeta potential, the entrapment efficiency and drug loading were measured by centrifugation. The release degree of naringenin chitosan nanoparticles was studied by constant temperature oscillating water bath. Finally, human lung cancer cell line A549 cells were used to carry out cytotoxicity and cell uptake. The results were: naringenin chitosan nanoparticles were spherical or spherical particles, and the structure was finished. The whole size is uniform, the sphericity is good, the PDI, the particle size, the Zeta potential and the entrapment efficiency are 0.268139 nm, +15.7 mV and 83.34% respectively. The release of naringenin nanoparticles is released in vitro, and the cumulative release of 24 h is over 80%. The release process in vitro is fitted with a good.MTT test with a good.MTT test to show the chitosan nanoparticles with different concentrations. After 72 h and cell action, the cell viability was more than 95%. The chitosan nanoparticles prepared in this paper had no cytotoxicity. The cell uptake test showed that after the action of FITC chitosan nanoparticles and A549 cells for 3 h, a large number of green fluorescent nanoparticles could be seen through the cell membrane into the cells. Conclusion: the ionic gel method has been successfully prepared by the ion gel method. The small naringenin chitosan nanoparticles have good slow release, small toxicity and high uptake rate of chitosan nanoparticles, which can greatly improve the utilization of drugs and have wide application prospects.
【作者单位】：锦州医科大学附属第三医院药剂科;
【基金】：辽宁省科技创新基金(编号:2014022037)
【分类号】：R943;R96

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