尼美舒利联合奥沙利铂对食管癌模型大鼠微血管密度及免疫功能的影响

发布时间：2018-06-27 11:31

本文选题：食管癌 + 大鼠　；参考：《中国药房》2017年04期

【摘要】：目的:研究尼美舒利联合奥沙利铂对食管癌模型大鼠微血管密度及免疫功能的影响。方法:取48只大鼠建立食管癌模型后随机分为模型组、奥沙利铂组、尼美舒利组和联用组,每组12只。建模后1 d,模型组大鼠尾iv 5%的葡萄糖注射液1 m L,每周3次,ig等量羧甲基纤维素钠溶液,每周1次;奥沙利铂组大鼠尾iv奥沙利铂13.6 mg/kg,每周3次;尼美舒利组大鼠ig尼美舒利20mg/kg,每周1次;联用组大鼠同法给予奥沙利铂和尼美舒利,给药时间为8周。观察各组大鼠肿瘤生长情况、肿瘤组织微血管密度、外周血免疫功能指标。结果:与模型组比较,各给药组大鼠肿瘤体积、瘤质量、肿瘤组织的积分吸光度和阳性血管密度均降低(P0.05);奥沙利铂组和联用组大鼠外周血CD3~+、CD4~+T细胞数和CD4~+/CD8~+比值均降低,CD8~+T细胞数均增加(P0.05)。与尼美舒利组比较,奥沙利铂组和联用组大鼠肿瘤体积、瘤质量、肿瘤组织的积分吸光度和阳性血管密度,以及外周血CD3~+、CD4~+T细胞数和CD4~+/CD8~+比值均降低,抑瘤率和CD8~+T细胞数均增加(P0.05),其中联用组较奥沙利铂组更明显(P0.05)。结论:奥沙利铂联合尼美舒利能够更有效地抑制食管癌的生长,其机制可能与抑制肿瘤组织中血管新生、增强免疫有关。
[Abstract]:Aim: to study the effects of nimesulide combined with oxaliplatin on microvessel density and immune function in esophageal carcinoma model rats. Methods: 48 rats were randomly divided into three groups: oxaliplatin group, nimesulide group and combination group. One day after modeling, the rats in the model group received 5% glucose injection of 1 mL of glucose 3 times a week, and oxaliplatin 13.6 mg / kg, 3 times a week. Nimesulide group rats were given 20 mg / kg nimesulide once a week, and rats in combination group were given oxaliplatin and nimesulide for 8 weeks. Tumor growth, tumor tissue microvessel density and peripheral blood immune function were observed. Results: compared with the model group, the tumor volume and tumor mass of rats in each administration group, The integral absorbance and positive vascular density of tumor tissue were decreased (P0.05), the number of CD3 ~ + CD4T cells and CD4 ~ / CD8- ~ ratio of peripheral blood in oxaliplatin group and combination group were all decreased (P0.05). Compared with nimesulide group, the tumor volume, tumor mass, integral absorbance and positive vessel density, the number of CD3 ~ + CD4T cells and the ratio of CD4 ~ / CD8 ~ in peripheral blood of oxaliplatin group and combination group were lower than those of nimesulide group. The inhibition rate and the number of CD8 ~ T cells were increased (P0.05), and the combination group was more obvious than oxaliplatin group (P0.05). Conclusion: oxaliplatin combined with nimesulide can inhibit the growth of esophageal carcinoma more effectively, and its mechanism may be related to the inhibition of angiogenesis in tumor tissues and the enhancement of immunity.
【作者单位】：武警山东省总队医院检验科;山东博科生物产业有限公司;
【分类号】：R965

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