羧甲基壳聚糖-聚乙二醇作为胰岛素载体的研究

发布时间：2018-06-28 08:31

本文选题：胰岛素 + 羧甲基壳聚糖　；参考：《中国医院药学杂志》2017年10期

【摘要】：目的:制备胰岛素-羧甲基壳聚糖-聚乙二醇纳米粒。方法:利用红外光谱(FTIR)和核磁共振氢谱(~1 H-NMR)对羧甲基壳聚糖-聚乙二醇的结构进行表征,用粒度分析仪测定纳米粒的粒径分布及电位,采用动态透析法考察纳米粒的释药性能,用CCK-8试剂盒检测纳米粒细胞毒性,以糖尿病小鼠为模型,研究纳米粒的降血糖作用。结果:聚乙二醇成功接枝到羧甲基壳聚糖上,包埋胰岛素的纳米粒的平均粒径为(257.5±12.1)nm,Zeta电位为(-15.2±0.3)mV,负载胰岛素的羧甲基壳聚糖-聚乙二醇纳米粒在中性释放介质中,5 h内胰岛素的释放速度较快,之后8 h趋于平稳,胰岛素的累计释放量可达到80%,CCK-8试剂盒显示纳米粒对L929细胞基本无细胞毒性,50 U·kg~(-1)的纳米粒溶液经灌胃给药后,血糖浓度明显降低。结论:胰岛素-羧甲基壳聚糖-聚乙二醇纳米粒基本无毒性,具有良好的生物相容性,对糖尿病小鼠有效发挥降血糖作用。
[Abstract]:Objective: to prepare insulin-carboxymethyl chitosan-polyethylene glycol nanoparticles. Methods: the structure of carboxymethyl chitosan-polyethylene glycol was characterized by FTIR and 1H-NMR. The particle size distribution and potential of nanoparticles were measured by particle size analyzer. The drug release properties of nanoparticles were investigated by dynamic dialysis. CCK-8 kit was used to detect the cytotoxicity of nanoparticles and the hypoglycemic effect of nanoparticles in diabetic mice was studied. Results: polyethylene glycol was grafted onto carboxymethyl chitosan. The average particle size of the imbedded insulin nanoparticles was (257.5 卤12.1) nm ~ (-1) nm Zeta potential was (-15.2 卤0.3) MV. The insulin release rate of the insulin loaded carboxymethyl chitosan / polyethylene glycol nanoparticles in neutral release medium was faster than that in the neutral release medium, and the insulin release rate tended to be stable after 8 hours. The cumulative release of insulin could reach 80% CCK-8 kit. The results showed that the concentration of blood glucose decreased significantly after oral administration of 50 U kg ~ (-1) nanoparticles with no cytotoxicity to L929 cells. Conclusion: insulin-carboxymethyl chitosan-polyethylene glycol nanoparticles are nontoxic, have good biocompatibility, and play an effective role in hypoglycemia in diabetic mice.
【作者单位】：武汉理工大学化学化工与生命科学学院;
【基金】：国家自然科学基金项目(编号:51273156)
【分类号】：R943

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