中空纤维离心超滤-HPLC法测定盐酸环丙沙星凝胶剂的含量

发布时间：2018-06-29 06:46

本文选题：盐酸环丙沙星凝胶剂 + 高效液相色谱法　；参考：《中国医院药学杂志》2017年11期

【摘要】：目的:建立中空纤维离心超滤的样品前处理技术结合HPLC法测定盐酸环丙沙星凝胶剂的含量。方法:采用中空纤维离心超滤技术对凝胶样品进行提取分离,基于中空纤维超滤膜特异性截留大分子原理,有效滤除了样品溶液中的高分子凝胶辅料卡波姆,超滤液直接进色谱分析。采用Promosil C_(18)柱(150×4.60 mm,5μm),流动相为甲醇-水(28∶72,含0.5%的三乙胺,用磷酸调节至pH4.0),检测波长为278 nm。结果:盐酸环丙沙星在2.51~25.1μg·mL-1范围内呈良好的线性关系(r=0.999 7),样品平均回收率为101.0%,RSD小于2%。结论:所建方法简便,快速,定量准确,色谱峰形良好,可用于盐酸环丙沙星凝胶剂的含量测定。
[Abstract]:Objective: to establish a sample pretreatment technique for the determination of ciprofloxacin hydrochloride gel with hollow fiber centrifugal ultrafiltration. Methods: hollow fiber centrifuge ultrafiltration technique was used to extract and separate the gel samples. Based on the principle of specific retention of macromolecules by hollow fiber ultrafiltration membrane, the polymer gel excipient Carbomer in the sample solution was effectively filtered. The ultrafiltration solution was analyzed by direct chromatography. A Promosil C _ (18) column (150 脳 4.60mm ~ (5) 渭 m),) was used. The mobile phase consisted of methanol-water (28: 72, containing 0.5% triethylamine, adjusted to pH 4.0 with phosphoric acid). The detection wavelength was 278nm. Results: the calibration curve of ciprofloxacin hydrochloride was linear in the range of 2.51U 25.1 渭 g mL-1 (r = 0.999 7). The average recovery of ciprofloxacin hydrochloride was 101.0% and RSD was less than 2%. Conclusion: the method is simple, rapid, accurate and has good chromatographic peak shape. It can be used for the determination of ciprofloxacin hydrochloride gel.
【作者单位】：中国石油天然气集团公司中心医院药学部;河北医科大学药学院;
【分类号】：O657.72;R927.2

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