衣霉素海马内给药诱导海马神经元死亡的新现象与假说

发布时间：2018-06-29 16:39

本文选题：衣霉素 + 红藻氨酸　；参考：《中国组织工程研究》2015年51期

【摘要】：背景:海马内注射衣霉素和红藻氨酸均可诱导内质网应激介导海马神经元凋亡,但两者大有不同。目的:比较海马CA1区内注射红藻氨酸和衣霉素后不同时间段海马神经元的死亡情况。方法:将96只成年雄性昆明小白鼠随机分为PBS组、红藻氨酸组和衣霉素组,海马CA1区分别注射红藻氨酸、衣霉素和PBS。结果与结论:Fluoro-Jade B染色显示,红藻氨酸组小白鼠注射后2,3,4,5,6,8 h损伤侧出现海马神经元死亡明显。衣霉素组小白鼠注射后4 h损伤侧CA1区可见神经元明显死亡,对侧无明显死亡;衣霉素组小白鼠注射后5 h损伤侧CA1-3区未见神经元明显死亡,但对侧CA1-3区可见大量神经元死亡。免疫组化染色显示,衣霉素组小白鼠注射后4,5 h神经元死亡明显区域内磷酸化真核翻译起始因子2α的表达明显上调。结果提示海马内注射衣霉素后5 h出现海马神经元同侧给药对侧死亡的现象,是由于海马内注射衣霉素后随着药物浓度的扩散在4.0-5.0 h间神经元凋亡可能有一个由同侧向对侧迅速蔓延的过程,这是不同于红藻氨酸海马内注射诱导神经元死亡过程的又一新现象。
[Abstract]:Background: Intra-hippocampal injection of either chlamycin or kainic acid can induce endoplasmic reticulum stress mediated apoptosis of hippocampal neurons. Aim: to compare the death of hippocampal neurons in hippocampal CA1 region after injection of kainic acid and chlortetracycline. Methods: 96 adult male Kunming mice were randomly divided into PBS group, kainic acid group and chlamycin group. The hippocampal CA1 region was injected with kainic acid, chlamycin and PBS, respectively. Results and conclusion the hippocampal neuronal death appeared in the injured side of kainic acid group at 8 h after injection of kainic acid. The neurons in the CA1 area of the injured side were obviously dead 4 hours after injection, but there was no obvious death in the contralateral CA1 area in the control group, but a large number of neurons in the CA1-3 region of the contralateral CA1-3 area were found to be dead in the CA1-3 region of the injured side at 5 h after injection in the chlortetracycline group. Immunohistochemical staining showed that the expression of phosphorylated eukaryote translation initiation factor 2 伪 was up-regulated in 4 h after injection. The results suggested that the ipsilateral death of hippocampal neurons occurred 5 hours after intrahippocampal administration of itamycin. This is because the apoptosis of neurons may spread rapidly from ipsilateral to contralateral with the diffusion of drug concentration within 4.0-5.0 h after intrahippocampal injection of itamycin. This is a new phenomenon which is different from the process of neuronal death induced by kainic acid in hippocampus.
【作者单位】：长沙医学院湖南省重点建设学科人体解剖与组织胚胎学教研室和重点实验室;湖南省人民医院脊柱外科;上海交通大学附属第六人民医院;湖南省人民医院心内科;
【基金】：国家自然科学基金项目(81171136) 湖南省大学生研究性学习和创新性实验计划项目(湘教通[2013]191号-431;湘教通[2012]402号-445) 湖南省高等学校科学研究项目重点项目(10A013) 湖南省高等学校“十二五”重点学科专项建设项目(湘教发[2011]76号)~~
【分类号】：R96

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