阿苯达唑亚砜温敏型原位凝胶抗泡球蚴的效果

发布时间：2018-06-30 06:11

本文选题：多房棘球绦虫 + 泡球蚴病　；参考：《中国人兽共患病学报》2017年08期

【摘要】：目的探索阿苯达唑亚砜(albendazole sulfoxide,ABZSO)与聚丙交酯乙交酯-聚乙二醇-聚丙交酯乙交酯(PLGA-PEG-PLGA)制备的阿苯达唑亚砜温敏型原位凝胶(ABZSO/PLGA-PEG-PLGA)抗泡球蚴的效果。方法应用ABZSO/PLGA-PEG-PLGA(20μg/mL、40μg/mL、80μg/mL)、ABZSO(40μg/mL)、PLGA-PEG-PLGA处理体外培养泡球蚴并统计原头节的存活率,采用扫描电镜观察各处理组泡球蚴或原头节形态变化;以各药物组治疗泡球蚴病大鼠动物模型后检查泡球蚴体积大小。结果药物处理体外培养泡球蚴18d时,ABZSO/PLGA-PEG-PLGA(40μg/mL)原头节存活率显著低于ABZSO(40μg/mL)组;扫描电镜结果显示ABZSO/PLGA-PEG-PLGA(80μg/mL)组的原头节结构被显著破坏;动物体内实验表明ABZSO/PLGA-PEG-PLGA(4mg/mL)组对抗泡球蚴效果比其他各药物组显著。结论 ABZSO/PLGA-PEG-PLGA具有抗泡球蚴的效果。
[Abstract]:Objective to investigate the effect of albendazole sulfoxide ABZSO and PLGA-PEG-PLGA on the antivesicular microspheres of albendazole sulfoxide thermo-sensitive in situ gel (ABZSOP / PLGA-PEG-PLGA). Methods ABZSO / PLGA-PEG-PLGA (20 渭 g / mL 40 渭 g / mL) and ABZSO (40 渭 g / mL) PLGA-PEG-PLGA were used to treat the cultured alveolar echinococci in vitro, and the survival rate of the original ganglion was counted. The morphological changes of alveolar globular or procephalus in each treatment group were observed by scanning electron microscope, and the size of alveolar ball was examined after the treatment of each drug group in the rat model of alveolar echinococcosis. Results the survival rate of ABZSO / PLGA-PEG-PLGA (40 渭 g / mL) was significantly lower than that of ABZSO / PLGA-PEG-PLGA (40 渭 g / mL) for 18 days, and the results of scanning electron microscope (SEM) showed that the structure of the original head was destroyed significantly in ABZSO / PLGA-PEG-PLGA (80 渭 g / mL) group. In vivo experiments showed that ABZSO / PLGA-PEG-PLGA (4 mg / mL) group had a better effect against alveolar hydatid than other drug groups. Conclusion ABZSO / P-PEG-PLGA has the effect of anti-alveolar hydatid.
【作者单位】：重庆医科大学病原生物学教研室重庆医科大学分子生物学与肿瘤研究中心;成都医学院病原生物学教研室;
【基金】：国家自然科学基金(No.30972567,81672045)资助~~
【分类号】：R96

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