1-甲基乙内酰脲抑制大鼠生长激素的研究

发布时间：2018-07-02 21:34

本文选题：1-甲基乙内酰脲 + 生长激素　；参考：《南华大学》2014年硕士论文

【摘要】：目的：探索1-甲基乙内酰脲能否降低生长激素水平，是否具有生长抑素类似物的功能，为非肽类生长抑素的研发提供实验基础及理论依据。方法：实验对象：雌性SD大鼠80只，体重230-250g。分组：将80只雌性SD大鼠按给药途径不同随机分为静脉组(V1-V5)和腹腔组（P1-P5）10组，每个大组设空白对照组（V1组、P1组）、造模对照组（V2组、P2组）、实验组（V3-V5组和P3-P5组），每组SD大鼠8只。处理：空白对照组（V1组、P1组）予以生理盐水0.5ml腹腔注射。造模对照组（V2组、P2组）予以10mg/ml苯巴比妥0.5ml腹腔注射造模。实验组先予以10mg/ml苯巴比妥0.5ml腹腔注射造模，10分钟后再对各个实验组按照不同给药途径及剂量给药：V3组、P3组注射10mg的1-甲基乙内酰脲0.5ml，V4组、P4组注射1mg的1-甲基乙内酰脲0.5ml，V5组、P5组注射0.1mg的1-甲基乙内酰脲0.5ml；空白对照组（V1组、P1组）、造模对照组（V2组、P2组）则分别给予PBS0.5ml。取样：各组均在注射生理盐水或者苯巴比妥造模后20分钟、30分钟、40分钟时由尾静脉取血，离心取上层血清。检测：按大鼠生长激素试剂盒说明做血清ELISA检测生长激素水平，对比各组之间生长激素水平变化。结果： 1.造模对照组较空白对照组的生长激素水平增高（P0.01）； 2.各个实验组较造模对照组生长激素水平降低（P0.01）； 3.静脉实验组（V3-V5组）组间比较：静脉注射1-甲基乙内酰脲10mg的V3组生长激素水平最低（P0.05），对SD大鼠生长激素的抑制效果最明显。 4.腹腔实验组（P3-P5组）组间比较：腹腔注射1-甲基乙内酰脲10mg的P3组生长激素水平最低（P0.05），，对SD大鼠生长激素的抑制效果最明显。 5.相同剂量的静脉实验组与腹腔实验组生长激素比较：静脉实验组的生长激素激素水平较腹腔实验组的生长激素水平低，V3组在用药后10分钟、20分钟（即造模后20分钟、30分钟）的生长激素水平低于P3组（P0.05），V4组在用药后10分钟、20分钟、30分钟（即造模后20分钟、30分钟、40分钟）的生长激素水平低于P4组（P0.05）。 6.造模组在造模后30分钟的生长激素水平最高，实验组（V3-V4组、P3-P5组）在用药后20分钟（即造模后30分钟）的生长激素水平最低，V3组、P3组用药后20分钟（即造模后30分钟）的生长激素水平低于用药后10分钟和30分钟（即造模后20分钟和40分钟）（P0.05），P4组、P5组用药后20分钟（即造模后30分钟）的生长激素水平低于用药后30分钟（即造模后40分钟）（P0.05）。结论： 1.1-甲基乙内酰脲能降低大鼠生长激素水平，在0.1mg~10mg之间有剂量依赖性，可能是一种新型的非肽类生长抑素。 2.1-甲基乙内酰脲注射后20分钟对大鼠生长激素的抑制效果最佳。 3.1-甲基乙内酰脲对大鼠生长激素的抑制效果静脉途径优于腹腔途径。
[Abstract]:Aim: to explore whether 1-methyl-acetonide can reduce growth hormone level and whether it has the function of somatostatin analogues, and provide experimental basis and theoretical basis for the development of non-peptide somatostatin. Methods: 80 female SD rats, weighing 230-250 g. Group: 80 female Sprague-Dawley rats were randomly divided into intravenous group (V1-V5) and peritoneal group (P1-P5). Each group was divided into blank control group (V1 group), model control group (V2 group P2 group), experimental group (V3-V5 group and P3-P5 group) with 8 SD rats in each group. Treatment: the blank control group (group V _ 1, P _ 1) was injected with normal saline 0.5ml intraperitoneally. 10mg/ml phenobarbital 0.5ml was injected intraperitoneally to model control group (group V 2, P 2). The experimental group was given 10mg/ml phenobarbital 0.5ml intraperitoneally for 10 minutes, then each experimental group was injected with 1-methyl-ethylglycolide (1-methylacetol urea-0.5ml) of 10mg in the P3 group according to different routes and doses of the drug, and then the 1-methylethylethyl of 1mg was injected into the P4 group. The 1-methyl-glycolylurea (1-methyl-glycolide) of 0.1mg was injected into the P5 group (0.5 ml) with 0.5 ml lactol urea (V5). The blank control group (V1 group) and the model control group (V2 group P 2 group) were given PBS 0.5 ml. Sampling: blood was collected from caudal vein 20 minutes after injection of normal saline or phenobarbital for 40 minutes after injection of phenobarbital. Detection: according to the instructions of rat growth hormone kit, serum Elisa was used to detect growth hormone level, and the changes of growth hormone level between groups were compared. Results: 1. The growth hormone level in the model control group was higher than that in the blank control group (P0.01); 2. The growth hormone level of each experimental group was lower than that of model control group (P0.01). Comparison of intravenous experimental group (V3-V5 group): intravenous injection of 1-methylacetol urea 10mg in V3 group had the lowest growth hormone level (P0.05), and the inhibitory effect on growth hormone in SD rats was the most obvious. 4. Comparison between the experimental group (P3-P5): the growth hormone level in P3 group was the lowest (P0.05), and the inhibitory effect on growth hormone in SD rats was the most obvious. 5. 5. Comparison of growth hormone levels between intravenous and celiac groups at the same dose: growth hormone levels in intravenous experimental group were lower than those in peritoneal experimental group. Group V3 was 20 minutes after administration of the drug (i.e. 20 minutes after modeling). The levels of growth hormone in the control group were lower than those in the P3 group (P0.05) and the growth hormone levels in the V4 group were significantly lower than those in the P4 group (P0.05) 10 minutes, 20 minutes, 30 minutes and 40 minutes after the treatment of the model (P < 0.05), and the level of growth hormone in the V4 group was significantly lower than that in the control group (P0.05). The growth hormone level of the model group was the highest at 30 minutes after modeling. Growth hormone levels in the experimental group (V3-V4 group P3-P5) were the lowest at 20 minutes (30 minutes after modeling). The levels of growth hormone in the V3 group were lower than those in the control group at 20 minutes (that is, 30 minutes after the establishment of the model) and were lower than those in the control group (10 minutes and 30 minutes after the treatment). The levels of growth hormone in P4 group (20 minutes and 40 minutes after model making) (P0.05) were lower than those in P4 group (P 0.05), and the levels of growth hormone in P4 group were lower than those in control group (P 0.05). Conclusion: 1.Methyl ethyl lactol urea can decrease the level of growth hormone in rats in a dose-dependent manner between 0.1mg~10mg. It may be a new type of non-peptide somatostatin. The inhibitory effect of 2.1-methyl-glycosylurea on growth hormone in rats is the best 20 minutes after injection. The inhibitory effect of 3.1-methyl-acetonide on growth hormone in rats The fruit vein approach was superior to the abdominal approach.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R965

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