阿托伐他汀联合L-4F对apoE缺失小鼠HDL功能及水平的影响

发布时间：2018-07-03 17:00

本文选题：阿托伐他汀 + 动脉粥样硬化　；参考：《重庆医学》2015年17期

【摘要】：目的通过采用高脂饮食喂养apoE缺失(apoE-/-)小鼠建立动脉粥样硬化(AS)模型,比较阿托伐他汀联合载脂蛋白A-I(apoA-I)模拟肽(L-4F)对AS小鼠血清氧磷酯酶(PON1)、髓过氧化物酶(MPO)活性及血清低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平的影响。方法雄性apoE-/-小鼠50只,高脂饲料喂养作为模型组,另外取适龄C57BL/6J小鼠20只给予普通饲料喂养作为正常对照组,实验4周后,处死2组小鼠各10只,检测相应血清指标,作为基线参考。其余apoE-/-小鼠分为模型对照组(单纯高脂饮食)、阿托伐他汀组(高脂饮食基础上给予阿托伐他汀治疗)、L-4F组(高脂饮食基础上给予L-4F治疗)、阿托伐他汀联合L-4F组(高脂饮食基础上给予阿托伐他汀+L-4F治疗),每组10只。实验第8周末处死5组小鼠,并取小鼠血清检测各血清指标。结果实验第8周末,与模型对照组比较,各药物组血清PON1活性显著升高(均P0.05),MPO活性显著下降(均P0.05);与模型对照组比较,阿托伐他汀组、阿托伐他汀联合L-4F组LDL-C水平显著减低(P0.05,P0.01),HDL-C水平显著升高(P0.05,P0.01),L-4F组LDL-C、HDL-C水平差异无统计学意义(均P0.05)。实验第8周末,阿托伐他汀联合L-4F组较阿托伐他汀组及L-4F组PON1活性、HDL-C水平显著升高(均P0.05);MPO活性、LDL-C水平显著降低(均P0.01)。结论阿托伐他汀、L-4F均可通过升高血清PON1活性,降低MPO活性,从而改善HDL抗氧化、抗炎功能,延缓AS进展。L-4F对LDL-C、HDL-C水平无影响。阿托伐他汀联合L-4F不仅降低LDL-C,升高HDL-C,而且更有效的改善HDL抗氧化、抗炎功能,从而延缓AS进展,优于单药使用。
[Abstract]:Objective to establish an atherosclerosis (as) model by feeding apoE deficient (apoE-r -) mice on a high-fat diet. To compare the effects of Atto vastatin combined with apolipoprotein A-I mimic peptide (L-4F) on serum phospholipase (PON1), myeloperoxidase (MPO) activity and serum low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) levels in as mice. Methods Fifty male apoE-r-mice were fed with high fat diet as model group, and 20 C57BL / 6J mice were fed with normal diet as normal control group. After 4 weeks of experiment, 10 mice in each group were killed and the corresponding serum indexes were detected. As a baseline reference. The remaining apoE-r-mice were divided into model control group (simple high-fat diet), Atto vastatin group (Atto vastatin treatment based on high-fat diet) and L-4F group (L-4F treatment based on high-fat diet), Atto vastatin combined with L-4F group (high fat diet). 10 rats in each group were treated with Atto vastatin L-4 F on the basis of fat diet. Five groups of mice were killed at the end of the 8th week of the experiment. Results at the end of the 8th week of the experiment, compared with the model control group, the serum PON1 activity of each drug group was significantly increased (P0.05), the activity of MPO was significantly decreased (P0.05), and compared with the model control group, the serum PON1 activity of each drug group was significantly higher than that of the model control group. The level of LDL-C in Atto combined with L-4F group was significantly decreased (P0.05, P0.01) and HDL-C level in L-4F group was significantly higher than that in L-4F group (P0.05). At the end of the 8th week, the level of PON1 activity and HDL-C in Atto combined with L-4F group was significantly higher than that in Atto group and L-4F group (P0.05). Conclusion Atto vastatin L-4F can increase the activity of serum PON1 and decrease the activity of MPO so as to improve the anti-oxidation and anti-inflammatory function of HDL.L-4F has no effect on the level of HDL-C. Atto vastatin combined with L-4F can not only decrease LDL-C and increase HDL-C, but also improve anti-oxidation and anti-inflammatory function of HDL-C more effectively, thus delaying the progress of as, which is better than that of single drug.
【作者单位】：南方医科大学南方医院心内科;中山大学附属第五医院内科;
【分类号】：R965

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