药物体外肝代谢的研究方法

发布时间：2018-07-04 13:00

本文选题：体外肝代谢 + 肝微粒体体外温孵法　；参考：《中国药房》2017年19期

【摘要】：目的:为药物体外肝代谢研究方法在新药研发中的应用提供参考。方法:以"药物代谢""体外肝代谢""肝微粒体体外温孵法""肝细胞体外温孵法""肝灌流技术""肝组织切片技术""基因重组P_(450)酶系""Drug metabolism""Liver metabolism in vitro""Metabolism of liver microsome in vitro""Liver perfusion technique""Liver biopsy technique""Recombination genetic cytochrome P_(450)"等为关键词,组合查询1996年10月-2017年4月在Pub Med、Web of Science、中国知网、中国生物医学等数据库中的相关文献,对体外肝代谢研究方法进行综述。结果与结论:共检索到相关英文文献220余篇、中文文献750余篇,其中有效文献30篇。常见的体外肝代谢研究方法有肝微粒体体外温孵法、肝细胞体外温孵法、肝灌流技术、肝组织切片技术、基因重组P_(450)酶系等。药物体外肝代谢不能全面反映体内药物的综合代谢情况,与体内的真实代谢情况存在差异,今后需结合体内实验等方法来完善药物在体内外的药物代谢转运研究;目前肝灌流技术和基因重组P_(450)酶系等体外肝代谢研究方法对设备、实验操作成本、数据处理技术等要求较高,其运用和推广仍然受到一定的约束和限制,今后需建立简单、快速、经济、高效的科学技术方法和手段。
[Abstract]:Objective: to provide reference for the application of drug in vitro liver metabolism research in the development of new drugs. Methods: drug metabolism, in vitro liver metabolism, liver microsomal incubation in vitro, liver cell incubation in vitro, liver perfusion technique, liver tissue section technique, recombinant P450 enzyme line, drug metabolism, liver metabolism in vitro, metabolism of liver microsome in vitro, liver perfusion technique, liver biopsy technique, Recombination Genetic cytochrome P450 ", etc. The research methods of in vitro liver metabolism were reviewed from October 1996 to April 2017 in the database of Pub Meden Web of Science, China knowledge Network, Chinese Biomedicine and so on. Results & conclusion: more than 220 English literatures and 750 Chinese literatures were retrieved, of which 30 were valid. The common methods for studying liver metabolism in vitro include liver microsomal incubation in vitro, hepatocyte incubation in vitro, liver perfusion technique, liver tissue section technique, recombinant P450 enzyme system and so on. Drug metabolism in vitro can not fully reflect the comprehensive metabolism of drugs in vivo, and there is a difference between in vivo and real metabolism. In the future, it is necessary to improve drug metabolism in vivo and in vitro by combining in vivo experiments. The in vitro liver metabolism research methods such as liver perfusion technique and recombinant P450 enzyme system require higher equipment, experimental operation cost, data processing technology, etc. Their application and popularization are still restricted and limited in some degree, so it is necessary to establish a simple method in the future. Rapid, economical and efficient scientific and technological methods and means.
【作者单位】：武汉大学人民医院药学部;武汉大学药学院;
【基金】：国家自然科学基金资助项目(No.31570349)
【分类号】：R96

【参考文献】