PEG包衣左氧氟沙星脂质体的制备及性质

发布时间：2018-07-05 09:20

本文选题：左氧氟沙星 + 肺靶向脂质体　；参考：《山东大学学报(医学版)》2017年08期

【摘要】：目的制备PEG2000包衣左氧氟沙星脂质体,并对其理化性质、初步稳定性及动力学特性进行评价。方法选用硫酸铵梯度法制备左氧氟沙星脂质体,以包封率及稳定性为指标筛选PEG2000包衣左氧氟沙星脂质体的最佳制备工艺;采用透射显微镜观察脂质体的外观形态;电位激光粒度分析仪测定粒径及表面Zeta电位;动态膜透析法测定脂质体体外释药特性;对其初步稳定性进行考察;对其体内药动学进行研究。结果 PEG2000包衣左氧氟沙星脂质体形态圆整,粒径分布均匀,包封率为(77.97±1.09)%,稳定性及体内外缓释性能均好于未包衣左氧氟沙星脂质体。结论构建的PEG包衣左氧氟沙星脂质体能明显改善脂质体的稳定性,能为提高脂质体的贮存稳定性开辟一条新途径。
[Abstract]:Aim to prepare PEG2000 coated levofloxacin liposome and evaluate its physicochemical properties, preliminary stability and kinetic properties. Methods Levofloxacin liposomes were prepared by ammonium sulfate gradient method. The optimum preparation process of PEG2000 coated levofloxacin liposomes was screened by using encapsulation efficiency and stability, and the appearance of levofloxacin liposomes was observed by transmission microscope. The particle size and surface Zeta potential of liposomes were measured by potentiometric laser particle size analyzer, the in vitro release characteristics of liposomes were determined by dynamic membrane dialysis, the preliminary stability of liposomes was investigated, and the pharmacokinetics of liposomes in vivo was studied. Results PEG2000 coated levofloxacin liposomes was round in shape and uniform in particle size distribution. The entrapment efficiency was (77.97 卤1.09). The stability and slow release properties of PEG2000 liposomes were better than those of uncoated levofloxacin liposomes. Conclusion PEG-coated levofloxacin liposomes can obviously improve the stability of liposomes and open up a new way to improve the storage stability of liposomes.
【作者单位】：山东大学药学院新药筛选平台;日照市人民医院妇产科;山东省环境保护科学研究设计院;山东大学附属省立医院普外科;山东大学药学院药物制剂研究所;
【分类号】：R943

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