液相色谱-质谱法和酶扩大免疫法测定地高辛血药浓度的方法研究

发布时间：2018-07-07 17:23

本文选题：地高辛 + 酶扩大免疫法　；参考：《中国临床药理学杂志》2016年07期

【摘要】：目的比较液相色谱-质谱法(LC-MS/MS)和酶扩大免疫法(EMIT)监测地高辛血药浓度的差异性。方法建立LC-MS/MS测定地高辛血药浓度的方法学。收集我院治疗药物监测室监测地高辛血浆标本75例,分别用LC-MS/MS和EMIT法进行测定,结果进行配对t检验。结果 LC-MS/MS测定地高辛血药浓度符合方法学要求。低质量浓度区域LC-MS/MS法与EMIT法差异无统计学意义(P0.05);中、高质量浓度区域EMIT法测定结果显著高于LC-MS/MS法(P0.001)。中质量浓度区域2种方法的平均差值为(0.57±0.72)ng·mL~(-1);高质量浓度区域的平均差值为(2.48±2.15)ng·mL~(-1),明显超出临床可接受范围。结论相比较于EMIT法,LC-MS/MS法能够排除血浆中免疫活性物质的干扰,实现地高辛血药浓度的准确定量。
[Abstract]:Objective to compare the difference between liquid chromatography-mass spectrometry (LC-MS) and enzyme expanded immunoassay (Emit) in monitoring digoxin concentration. Methods A method for the determination of digoxin in blood by LC-MS / MS was established. A total of 75 plasma samples of digoxin were collected from our hospital. The plasma samples were measured by LC-MS / MS and Emit respectively. The results were tested by paired t test. Results determination of digoxin in blood by LC-MS / MS met the requirements of methodology. There was no significant difference between LC-MS / MS method and Emit method in low concentration region (P0.05), and the results of Emit method in high concentration region were significantly higher than that of LC-MS / MS method (P0.001). The average difference between the two methods was (0.57 卤0.72) ng mL ~ (-1) and (2.48 卤2.15) ng mL ~ (-1), which was obviously beyond the range of clinical acceptance. Conclusion compared with Emit method, LC-MS / MS method can eliminate the interference of immunoreactive substances in plasma and achieve accurate quantification of plasma digoxin concentration.
【作者单位】：河北省人民医院药学部;
【基金】：河北省医学适用技术跟踪基金资助项目(G2015006) 河北省卫计委医学科学研究重点课题基金资助项目(20130409)
【分类号】：R927.2;O657.63

【相似文献】