洛铂对宫颈癌SiHa细胞射线敏感性的影响

发布时间：2018-07-08 10:48

本文选题：洛铂 + 射线敏感性　；参考：《中国临床药理学杂志》2017年13期

【摘要】：目的研究洛铂对宫颈癌SiHa细胞射线敏感性的影响及其机制。方法 MTT法检测不同浓度洛铂对SiHa细胞的作用24,48和72 h后的增殖情况,计算IC_(50)值。单克隆形成实验检测洛铂对SiHa细胞的射线敏感性影响,单击多靶模型拟合SiHa细胞的放射生物学参数。基于MTT法和细胞克隆形成实验结果,将细胞分为空白组(不加任何药物)和洛铂组(5.0μmol·L~(-1))、放疗组(6 Gy 6 MV X线)和洛铂+放疗组(5.0μmol·L~(-1)洛泊+6 Gy 6 MV X线)。流式细胞术检测洛铂、射线以及洛铂联合射线对SiHa细胞凋亡率的影响。Western blot检测促凋亡蛋白Bax和抑凋亡蛋白Bcl-2在SiHa细胞中的表达。结果空白组、洛铂组、放疗组和洛铂联合放疗组的凋亡率分别为(4.44±2.07)%,(30.50±3.17)%,(43.08±5.93)%和(84.68±4.90)%。洛铂组、放疗组相对空白组促凋亡蛋白Bax表达显著上调,抑凋亡蛋白Bcl-2显著下调。洛铂联合放疗组相对洛铂组、放疗组进一步显著上调Bax和下调Bcl-2的表达。结论洛铂可能通过细胞凋亡途径增强SiHa细胞射线敏感性。
[Abstract]:Objective to study the effect of loplatin on radiosensitivity of cervical cancer SIHA cells and its mechanism. Methods MTT assay was used to detect the proliferation of SIHA cells treated with different concentrations of loplatin for 24 h and 72 h, and the IC50 values were calculated. The effects of roplatin on radiosensitivity of SIHA cells were detected by monoclonal forming assay. The radiobiological parameters of SIHA cells were fitted by clicking on a multi-target model. Based on the results of MTT assay and cell clone formation assay, the cells were divided into three groups: blank group (without any drugs), loplatin group (5.0 渭 mol L ~ (-1), radiotherapy group (6 Gy 6 MV X ray) and loplatin radiotherapy group (5.0 渭 mol L ~ (-1) lopoplatin 6 Gy 6 MV X ray). The apoptosis rate of SIHA cells was detected by flow cytometry. Western blot was used to detect the expression of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2 in SIHA cells. Results the apoptotic rates were (4.44 卤2.07), (30.50 卤3.17), (43.08 卤5.93)% and (84.68 卤4.90) in blank group, loplatin group, radiotherapy group and combined radiotherapy group, respectively. The expression of pro-apoptotic protein Bax was up-regulated and the anti-apoptotic protein Bcl-2 was significantly down-regulated in the Lopplatin group and the radiotherapy group compared with the blank group. The expression of Bax and Bcl-2 were further up-regulated and down-regulated in the Lopplatin combined with radiotherapy group as compared with that in the Lopplatin group. Conclusion Lopplatin may enhance radiosensitivity of SIHA cells through apoptosis.
【作者单位】：西南医科大学药学院;内江市第二人民医院;
【分类号】：R96

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