蛋氨酸锌在大鼠体内的药代动力学和组织分布研究

发布时间：2018-07-10 01:59

本文选题：蛋氨酸锌 + 药代动力学　；参考：《卫生研究》2015年05期

【摘要】：目的研究蛋氨酸锌(Zn-Met)在大鼠体内的药代动力学和组织分布。方法大鼠灌胃2 mg Zn/kg Zn-Met,于0.5、1、2、3、4、6、8、12和24 h后采集血样;另设其它组别大鼠,灌胃2 mg Zn/kg Zn-Met,于1、8和24 h后采集心、肝、脾、肺和肾组织样品。大鼠血浆样品用0.5%硝酸溶液稀释处理,组织样品采用硝酸与高氯酸混合酸消化处理。采用电感耦合等离子体质谱(ICP-MS)测定大鼠血浆和组织中锌浓度,非房室模型计算Zn-Met的动力学参数。结果 ICP-MS测定锌的方法定量限为1 ng/ml,准确度在93.2%~97.8%,精密度在4.5%以内。大鼠灌胃Zn-Met的末端消除半减期(t1/2,λ)为(9.47±1.85)h,表观分布容积(V)为(1101±154)ml/kg,清除率(CL)为(80.53±9.26)ml/(h·kg)。结论 Zn-Met在大鼠体内吸收较快,2 h达到最大血药浓度,消除缓慢,广泛分布于大鼠各组织中,其中肝中浓度最高,其次为脾。
[Abstract]:Objective to study the pharmacokinetics and tissue distribution of zinc methionine (Zn-Met) in rats. Methods Blood samples were collected from rats at the dose of 2 mg Zn / kg Zn-Met.Blood samples were collected from rats at 0.5 mg Zn / kg Zn-Met.The samples of heart, liver, spleen, lung and kidney were collected at 1h and 24h after intragastric administration of 2mg Zn / kg Zn-Met.Blood samples were collected from rats at 12 and 24 hours after administration of 2 mg Zn / kg Zn-Met.The rats were fed with Zn-Metals at the dose of 2 mg Zn / kg for 12 h and 24 h, respectively. Rat plasma samples were diluted with 0.5% nitric acid solution and tissue samples were digested with mixed acid of nitric acid and perchloric acid. The concentration of zinc in plasma and tissue of rats was determined by inductively coupled plasma mass spectrometry (ICP-MS) and the kinetic parameters of Zn-Met were calculated by non-atrioventricular model. Results the limit of determination of zinc by ICP-MS was 1 ng / ml, the accuracy was 93. 2% and 97. 8%, and the precision was less than 4. 5%. The end elimination half reduction period (t 1 / 2, 位) of Zn-Met was (9.47 卤1.85) h, the apparent distribution volume (V) was (1101 卤154ml / kg), and the clearance rate (CL) was (80.53 卤9.26) ml/ (h kg). Conclusion Zn-Met can reach the maximum concentration in blood at 2 h after absorption in rats, and its elimination is slow. Zn-Met is widely distributed in various tissues of rats, among which the highest concentration is in the liver and the second is in the spleen.
【作者单位】：山东省即墨市人民医院;
【分类号】：R965

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