钙调磷酸酶抑制剂筛选方法的研究进展

发布时间：2018-07-11 13:29

  本文选题：钙调磷酸酶 + 抑制剂　； 参考：《中国药理学与毒理学杂志》2017年04期

【摘要】：筛选和开发新型低毒的钙调磷酸酶抑制剂(CNI)宜扬长避短,充分利用各方法的优势。本文综述了酵母"正筛选"模型,酵母报告基因高通量CNI筛选模型和钙调磷酸酶(Ca N)活性检测法3种常用的筛选方法的适用范围、优缺点以及多方法联合。其中,酵母"正筛选"模型利用生长圈来代替抑菌圈,有效避免了抑菌化合物造成的"假阳性";酵母报告基因的高通量筛选模型有较高的筛选效率,适合于大量样品的初步筛选;Ca N活性检测法具有作用靶点明确和抑制效率直观准确的优点。本文旨在为筛选和开发新型、低毒的CNI提供参考。
[Abstract]:Screening and developing a new type of low toxicity calmodulase inhibitor (CNI) should make full use of the advantages of each method. In this paper, the application, advantages and disadvantages of three common screening methods, including yeast "positive screening" model, yeast reporter gene high-throughput CNI screening model and calcium phosphatase (Ca _ N) activity test, were reviewed. Among them, yeast "positive screening" model uses growth circle instead of bacteriostasis circle, which effectively avoids "false positive" caused by bacteriostatic compounds, and the high-throughput screening model of yeast reporter gene has higher screening efficiency. The primary screening method for the detection of Ca N activity is suitable for a large number of samples. It has the advantages of clear targets and direct and accurate inhibition efficiency. The purpose of this paper is to provide a reference for screening and developing new, low-toxicity CNI.
【作者单位】： 浙江工业大学生物工程学院;
【分类号】：R91
，

本文编号：2115356