塞来昔布对颅脑创伤后Caspase-9表达及运动功能的影响

发布时间：2018-07-12 17:00

本文选题：塞来昔布 + 颅脑损伤　；参考：《中国药理学通报》2017年06期

【摘要】：目的探讨塞来昔布对大鼠颅脑创伤后环氧合酶(COX-2)和凋亡蛋白Caspase-9表达及运动功能的影响。方法实验分为对照组、假手术组、创伤组、治疗组,采用Marmarou方法建立大鼠闭合性颅脑创伤模型,实时荧光定量PCR(q PCR)检测COX-2和Caspase-9基因表达量,免疫组织化学染色法检测COX-2和Caspase-9蛋白的表达,神经功能损害评分(NSS)检测大鼠的运动功能。结果创伤组COX-2和Caspase-9基因和蛋白的表达明显高于其他3组(P0.05),治疗组较创伤组能有效降低COX-2和Caspase-9的表达,但仍高于假手术组和正常组(P0.05);与创伤组相比较,治疗组能有效改善大鼠的运动功能障碍(P0.05),和对照组和假手术组比较差异具有统计学意义(P0.05)。结论塞来昔布可通过对COX-2的特异性抑制作用,减少颅脑损伤后炎症反应,并进一步降低Caspase-9的表达,从而减少神经细胞的凋亡,并改善大鼠颅脑创伤后的运动功能障碍。
[Abstract]:Objective to investigate the effect of celecoxib on the expression of cyclooxygenase (COX-2) and apoptosis protein Caspase-9 after traumatic brain injury in rats. Methods the experimental group was divided into control group, sham operation group, trauma group and treatment group. The model of closed craniocerebral trauma was established by Marmarou method, and real-time fluorescence quantitative PCR (Q PCR) was used to detect COX-2 and Caspase-9 base. The expression of COX-2 and Caspase-9 protein was detected by immunohistochemical staining, and the motor function of rats was detected by nerve function impairment score (NSS). Results the expression of COX-2 and Caspase-9 gene and protein in the trauma group was significantly higher than that of the other 3 groups (P0.05). The treatment group was more effective than the trauma group to reduce the expression of COX-2 and Caspase-9, but it was still higher than that in the trauma group. The sham operation group and the normal group (P0.05), compared with the trauma group, the treatment group could effectively improve the motor dysfunction (P0.05) in the rats (P0.05). Conclusion the celecoxib could reduce the inflammatory reaction after the brain injury and further reduce the Caspas through the specific suppression of COX-2. The expression of e-9 can reduce neuronal apoptosis and improve motor dysfunction after traumatic brain injury in rats.
【作者单位】：河北医科大学附属邢台市人民医院神经外科;邢台医学高等专科学校药学系;华北理工大学基础医学院;
【基金】：河北省自然科学基金项目(No C2004000689) 河北省博士基金项目(No 05547008D-4) 河北省科学技术与社会发展计划项目(No 04276135)
【分类号】：R965

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