枣环磷酸腺苷提取液对小鼠抗疲劳作用的药效学研究

发布时间：2018-07-13 20:53

【摘要】：目的:探讨枣环磷酸腺苷提取液对啮齿类动物的抗疲劳作用,为临床试验提供数据支持。方法:1将40只小鼠随机分为模型组、对照组(口服红景天液体)和枣环磷酸腺苷提取液(2.2、6.7和20.0 ml/kg)组。环磷酸腺苷剂量为0.42、1.28和3.84 mg/kg,分别连续口服14 d,每天1次。采用Morris水迷宫强迫小鼠游泳,记录小鼠30 min内的游泳划水时间,以评价其抗疲劳效果;2再将40只大鼠随机分为模型组、对照组(口服红景天液体)和枣环磷酸腺苷提取液(1.1、3.3和10.0 ml/kg)组。环磷酸腺苷剂量为0.21、0.64和1.92 mg/kg,分别连续口服14 d,每天1次。采用Morris水迷宫使各组大鼠强迫游泳20 min,检测大鼠强迫游泳后的血清生化和肝组织糖原水平。结果:枣环磷酸腺苷提取液中、高剂量组动物的游泳划水时间与模型组相比有统计学意义;三个不同剂量的枣环磷酸腺苷提取液对游泳后动物的血清尿素氮含量均有明显的降低作用,与模型组相比有统计学意义,中、高剂量组动物的血清乳酸明显下降,同时高剂量组肝组织糖原含量显著增加,枣环磷酸腺苷提取液对各组动物乳酸脱氢酶没有影响。结论:枣环磷酸腺苷提取液具有较好的抗疲劳和增强机体耐力的作用。
[Abstract]:Objective: to study the anti-fatigue effect of jujube cyclic adenosine phosphate extract on rodents and to provide data support for clinical trials. Methods 40 mice were randomly divided into model group, control group (oral salidroside liquid) and jujube cyclic adenosine monophosphate extract (2.2 ml/kg and 20.0 ml/kg). The dosages of cyclic adenosine monophosphate were 0.42mg / kg and 3.84 mg / kg, respectively, once a day for 14 days. Morris water maze was used to force the mice to swim. The swimming stroke time of the mice was recorded within 30 min, and then 40 rats were randomly divided into model group. The control group (oral salidroid liquid) and jujube cyclic adenosine phosphate extract (1.1 ml/kg and 10.0 ml/kg) group. The dosages of cyclic adenosine monophosphate were 0.21 渭 g / kg and 1.92 mg / kg, respectively, once a day for 14 days. Morris water maze was used to force swimming for 20 min. Serum biochemistry and liver glycogen level were measured after forced swimming. Results: in the jujube cyclic adenosine monophosphate extract, the swimming stroke time of the high dose group was significantly different from that of the model group. Three different doses of jujube cyclic adenosine monophosphate (jujube cyclic adenosine monophosphate) extract significantly decreased the serum urea nitrogen content after swimming, compared with the model group, the serum lactic acid of the middle and high dose groups decreased significantly. At the same time, the content of glycogen in liver tissue of high dose group was significantly increased, and the extract of jujube cyclic adenosine phosphate had no effect on lactate dehydrogenase. Conclusion: jujube cyclic adenosine phosphate extract has better anti-fatigue and endurance effects.
【作者单位】：北京武警总医院计划科;武警医学院附属医院营养科;武警总医院医学实验中心;中恩医药科技有限公司;
【分类号】：R965

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