1-甲基乙内酰脲皮下注射及胃饲降低大鼠生长激素水平的实验

发布时间：2018-07-15 22:36

【摘要】：目的：探索1-甲基乙内酰脲不同给药途径、给药剂量对降低大鼠生长激素的影响；探究胃饲是否同样具有降低生长激素的作用。方法：实验对象：成年雌性SD大鼠80只。分组：将80只雌性SD大鼠按给药途径不同随机分为皮下注射组(H1-H5)和胃饲组（G1-G5）两个大组、10个小组，每小组大鼠8只；每个大组设空白对照组（H1组、G1组）、造模对照组（H2组、G2组）、实验组（H3-H5组和G3-G5组）。处理：空白对照组（H1组、G1组）予以生理盐水0.5ml腹腔注射。造模对照组（H2组、G2组）予以10mg/ml苯巴比妥0.5ml腹腔注射造模。实验组先予以10mg/ml苯巴比妥0.5ml腹腔注射造模，造模后10分钟再对各个实验组按照不同给药途径及剂量给药：H3组、G3组给予1-甲基乙内酰脲0.1mg，H4组、G4组给予1-甲基乙内酰脲1.0mg，H5组、G5组给予1-甲基乙内酰脲10mg）；空白对照组（H1组、G1组）、造模对照组（H2组、G2组）、造模后10分钟，则分别给予PBS0.5ml。取样：各组均在注射生理盐水或苯巴比妥造模后20分钟、30分钟、40分钟时由尾静脉取血，离心取上层血清。检测：按大鼠生长激素试剂盒说明做血清ELISA检测生长激素水平，对比各组之间生长激素水平变化。结果分析：应用计算软件SPSS18.0，统计分析得出结果。结果： 1.造模对照组较空白对照组大鼠生长激素水平增高显著，空白对照组腹腔注射生理盐水后30分钟生长激素水平为5.022±0.469ug/L。造模对照组的造模后30分钟生长激素水平为10.00±0.748ug/L，两组生长激素值差异有统计学意义（P0.05），提示造模成功。 2.皮下注射组可以有效降低大鼠生长激素的水平。皮下注射1-甲基乙内酰脲10mg组20分钟（即造模后30分钟），生长激素水平为2.960±0.202ug/L。皮下注射1-甲基乙内酰脲1mg组及0.1mg组均可降低大鼠生长激素的水平，与造模对照组比较生长激素显著降低，差异均具有统计学意义（P0.05），提示皮下注射1-甲基乙内酰脲可显著降低大鼠生长激素的水平。 3.胃饲组可有效降低大鼠生长激素的水平；胃饲1-甲基乙内酰脲10mg/只组30分钟（即造模后40分钟），生长激素水平为4.212±0.525ug/L。胃饲1-甲基乙内酰脲1mg组及0.1mg组均可降低大鼠生长激素的水平，，与造模对照组比较生长激素显著降低，差异均具有统计学意义（P0.05），提示胃饲1-甲基乙内酰脲可显著降低大鼠生长激素的水平。 4.皮下注射效果要优于胃饲组皮下注射组：皮下注射1-甲基乙内酰脲10mg降低大鼠生长激素的最低值2.960±0.202ug/L，胃饲1-甲基乙内酰脲10mg降低大鼠生长激素最低值4.212±0.525ug/L，两组生长激素值差异有统计学意义（P0.05），提示皮下注射1-甲基乙内酰脲优于胃饲。 5.皮下注射组降低大鼠生长激素的最低值在用药后20分钟（即造模后30分钟），而胃饲组在用药后30分钟（即造模后40分钟）时间段的大鼠生长激素的下降最明显。结论： 1.皮下注射及胃饲1-甲基乙内酰脲均具有降低大鼠生长激素水平的作用； 2.皮下注射1-甲基乙内酰脲降低大鼠生长激素效果优于胃饲；
[Abstract]:Objective:
To explore the different routes of administration of 1- methylhydantoin and the effect of dosage on the growth hormone of rats; explore whether gastric feeding also has the effect of reducing growth hormone.
Method:
Subjects: 80 adult female SD rats were divided into groups: 80 female SD rats were randomly divided into subcutaneous injection group (H1-H5) and stomach feeding group (G1-G5) in two large groups, 10 groups and 8 rats in each group; each group had blank control group (group H1, G1), model control group (H2 group, G2 group), experimental group (H3-H5 group and G3-G5 group). Treatment: the blank control group (group H1, group G1) was given 0.5ml intraperitoneal injection of saline 0.5ml. The model control group (group H2, G2) was injected with 10mg/ml phenobarbital 0.5ml intraperitoneal injection molding. The experimental group first gave 10mg/ml phenobarbital intraperitoneal injection molding, and then 10 minutes after the model, the various experimental groups were given different ways and dosages: H3 group, G3. Group 1- methylhydantoin 0.1mg, group H4, group G4 given 1- methylhydantoin 1.0mg, H5 group, G5 group giving 1- methyl hydantoin 10mg); blank control group (H1 group, G1 group), model control group, 10 minutes after modeling, respectively, for 20 minutes after injection of saline or phenobarbital, 30 In minutes, the blood was taken from the tail vein at 40 minutes, and the upper serum was centrifuged. The level of growth hormone was detected by the rat growth hormone kit, and the level of growth hormone was compared with the growth hormone level between each group. The results were analyzed by the application of computing software SPSS18.0, and the results were obtained by statistical analysis.
Result:
1. the growth hormone level in the control group was significantly higher than that in the blank control group. The growth hormone level of the control group was 10 + 0.748ug/L after the 30 minute model of the control group with the growth hormone level of 5.022 + 0.469ug/L. after intraperitoneal injection of saline (P0.05), and the difference of growth hormone values in the two groups was statistically significant (P0.05). Success.
2. subcutaneous injection group can effectively reduce the level of growth hormone in rats. Subcutaneous injection of 1- methylhydantoin 10mg group 20 minutes (i.e. 30 minutes after modeling), growth hormone level of 2.960 + 0.202ug/L. subcutaneous injection of 1- methyl hydanreon 1mg group and 0.1mg group can reduce the level of rat growth irritable, compared with the model control group, the growth of Ji Suxian was compared with the model control group. The difference was statistically significant (P0.05), suggesting that subcutaneous injection of 1- methylhydantoin could significantly reduce the level of growth hormone in rats.
3. gastric feeding group can effectively reduce the level of growth hormone in rats; gastric feeding 1- methylhydantoin 10mg/ is only 30 minutes (i.e. 40 minutes after modeling), growth hormone level is 4.212 + 0.525ug/L. gastric feeding 1- methyl hydantoin 1mg group and 0.1mg group can reduce the level of growth hormone in rats, compared with the model control group, the growth hormone is significantly lower than that in the model control group. The difference was statistically significant (P0.05), suggesting that gastric feeding of 1- methylhydantoin could significantly reduce the level of growth hormone in rats.
4. the effect of subcutaneous injection was better than that in the subcutaneous injection group of the stomach feeding group: the minimum value of 1- methylhydantoin 10mg subcutaneous injection was 2.960 + 0.202ug/L, and the lowest growth hormone of the gastric feeding 1- methylhydantoin 10mg was reduced by 4.212 + 0.525ug/L, and the difference of growth hormone value was statistically significant (P0.05), suggesting subcutaneous injection of 1-. Methylhydantoin is superior to gastric feeding.
5. the lowest value of the growth hormone in the subcutaneous injection group was 20 minutes after the medication (30 minutes after the model was made), while the growth hormone of the rats in the stomach feeding group was the most obvious at 30 minutes after the medication (that is, 40 minutes after the model 40 minutes).
Conclusion:
1. subcutaneous injection and gastric feeding of 1- methylhydantoin can reduce the growth hormone level in rats.
2. the effect of subcutaneous injection of 1- methylhydantoin on growth hormone in rats was better than that in gastric feeding.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R965

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