内毒素亲和吸附剂对创伤失血性休克大鼠肠损害及炎症因子水平的影响

发布时间：2018-07-16 08:25

【摘要】：目的研究内毒素亲和吸附剂(EAA)对创伤失血性休克大鼠肠损害及炎症因子水平的影响。方法选取SD大鼠,按照体重随机分为5组:正常组、模型组和低中高3个剂量实验组,每组10只。通过创伤和放血构建创伤失血性休克大鼠模型。在建模前30 min,低中高3个剂量(EAA 5,10,15 m L+15,10,5m L 0.9%Na Cl)实验组分别于大鼠消化道内灌注。用HE染色法观察大鼠肠道绒毛形态变化以及肠黏膜损伤程度。复苏后4,8,16 h对大鼠的内毒素、一氧化氮(NO)、肿瘤坏死因子α(TNF-α)水平进行检测。结果与空白组0.03±0.01比较,模型组的肠黏膜损伤指数为5.41±2.56,差异有统计学意义(P0.05)。复苏4 h后,与模型组比较,高、中、低3个剂量实验组的大鼠肠黏膜损伤指数分别为3.45±0.75,2.53±0.68,2.02±0.53,差异均有统计学意义(均P0.05)。与空白组比较,模型组大鼠的血清内毒素、NO、TNF-α水平分别为(456.52±21.56)pg·m L~(-1),(108.41±9.56)ng·m L~(-1),(77.58±10.65)ng·m L~(-1),差异均有统计学意义(均P0.05)。复苏4 h后,高、中、低3个剂量实验组的大鼠内毒素、NO、TNF-α的水平分别为(280.41±15.42),(254.63±17.56),(221.53±13.53)pg·m L~(-1);(90.43±8.42),(63.54±7.53),(49.52±8.41)ng·m L~(-1);(77.58±10.65),(62.58±5.12),(42.51±4.53)ng·m L~(-1)。与模型组比较,3个剂量实验组的大鼠内毒素、NO水平差异均有统计学意义(均P0.05);而TNF-α水平只有中低2个剂量实验组差异均有统计学意义(均P0.05)。结论低浓度EAA能够有效缓解失血性休克引起的应激反应,降低炎症因子水平,从而保护大鼠肠功能。
[Abstract]:Objective to study the effects of endotoxin affinity adsorbent (EAA) on intestinal damage and inflammatory factors in rats with traumatic hemorrhagic shock. Methods Sprague-Dawley rats were randomly divided into 5 groups according to their body weight: normal group, model group and low, medium and high dose groups, with 10 rats in each group. The rat model of traumatic hemorrhagic shock was established by trauma and bloodletting. 30 minutes before modeling, the experimental group was perfused in the digestive tract of rats at three doses (EAA 5: 1015m / L, 1015m / L, 105m / L) and NaCl (0.9m / L), respectively. The morphological changes of intestinal villi and the degree of intestinal mucosal injury were observed by HE staining. The levels of endotoxin, nitric oxide (no) and tumor necrosis factor- 伪 (TNF- 伪) in rats were measured at 4 and 8 hours after resuscitation. Results compared with the blank group (0.03 卤0.01), the intestinal mucosal injury index in the model group was 5.41 卤2.56, the difference was statistically significant (P0.05). After 4 hours of resuscitation, compared with the model group, the intestinal mucosal injury index of the experimental groups with high, middle and low doses was 3.45 卤0.75V 2.53 卤0.68 卤2.02 卤0.53, respectively. The difference was statistically significant (P0.05). Compared with the blank group, the serum levels of TNF- 伪 in the model group were (456.52 卤21.56) pg m / L ~ (-1), (~ (-1) 卤9.56) ng m L ~ (-1), (77.58 卤10.65) ng m L ~ (-1), respectively (P0.05). 4 h after resuscitation, the levels of TNF- 伪 were (280.41 卤15.42), (卤17.53) pg m L-1); (90.54 卤7.53), (49.52 卤8.41) ng m L-1); (77.58 卤10.65), (62.58 卤5.12), (42.51 卤4.53) ng m L ~ (-1) respectively) in the three experimental groups. Compared with the model group, the levels of endotoxin and no in the three dose groups were significantly different (P0.05), while the levels of TNF- 伪 in the middle and low dose groups were statistically significant (P0.05). Conclusion low concentration EAA can effectively relieve the stress response induced by hemorrhagic shock and reduce the level of inflammatory factors, thereby protecting the intestinal function of rats.
【作者单位】：开滦总医院麻醉科;
【基金】：河北省医学科学研究重点课题计划基金资助项目(ZD20140064)
【分类号】：R965

【相似文献】