光谱法研究三磷酸腺苷二钠与牛血清白蛋白的相互作用及共存金属离子的影响

发布时间：2018-07-21 09:03

【摘要】：在模拟人体生理条件下,用紫外-可见吸收光谱法和荧光光谱法研究三磷酸腺苷二钠(ADT)和牛血清白蛋白(BSA)的相互作用及共存金属离子Mn~(~(2+)),Co~(2+),Cr~(3+),Mg~(2+),Fe~(3+)对两者结合作用的影响.结果表明:ADT对BSA的荧光有猝灭作用,其猝灭过程属于动态猝灭.通过计算得出ADT与BSA的结合常数Kb及结合为点数n.根据热力学参数确定了ADT和BSA之间的作用力类型主要为静电引力.ADT在BSA中的结合位点主要位于亚螺旋域ⅡA.Hill系数nHill≈1,表明ADT对两者的结合几乎无协同作用.用同步荧光光谱法研究了ADT对BSA构象的影响.此外还详细探讨了共存金属离子对ADT与BSA结合作用的影响.
[Abstract]:The interaction of adenosine triphosphate disodium (ADT) with bovine serum albumin (BSA) and the effect of coexisting metal ions mn ~ (2) C ~ (2) C _ (2) C _ (2) mg ~ (2) Fe ~ (3) on the interaction between ADT and bovine serum albumin (BSA) were studied by UV-Vis absorption spectroscopy and fluorescence spectroscopy under simulated human physiological conditions. The results show that the fluorescence of BSA is quenched by: 1. The quenching process is dynamic. The binding constant of ADT and BSA was calculated, and the binding point was n. According to the thermodynamic parameters, the interaction force between ADT and BSA is mainly composed of electrostatic force. The binding site of ADT in BSA is mainly located in the subhelical region 鈪，

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