微柱凝胶联合高效液相色谱法同时测定双载药脂质体中阿霉素与他莫昔芬的包封率

发布时间：2018-07-26 10:44

【摘要】：目的:建立双载药脂质体中阿霉素(DOX)与他莫昔芬(TAM)包封率的测定方法。方法:采用Sephadex LH-20分离载药脂质体与游离药物。采用HPLC法梯度洗脱,测定脂质体中装载药物的含量及脂质体制备时加入的药物总量,计算脂质体中DOX与TAM的包封率。结果:在所选择的色谱条件下,DOX与TAM的专属性良好。DOX与TAM分别在4.68~37.44,4.80~38.40μg·mL-1范围内线性关系良好。流出曲线研究结果表明,Sephadex LH-20微柱法能有效分离载药脂质体与未包载的游离药物,游离药物DOX与TAM的柱回收率分别为98%~102%、99%~101%。微柱凝胶分离-HPLC法测定得,双载药脂质体中DOX与TAM的含量均约为1 mg·mL-1,包封率均90%。结论:该方法简便快速、精密可靠,可应用于双载药脂质体中DOX与TAM含量与包封率的测定。
[Abstract]:Aim: to establish a method for the determination of the entrapment efficiency of adriamycin (DOX) and tamoxifen (TAM) in double-drug-loaded liposomes. Methods: liposomes and free drugs were separated by Sephadex LH-20. HPLC gradient elution was used to determine the content of the drugs loaded in liposomes and the total amount of drugs added in the preparation of liposomes. The encapsulation efficiency of DOX and TAM in liposomes was calculated. Results: under the selected chromatographic conditions, the specificity of dox and TAM was good. The linear relationship between dox and TAM was in the range of 4.68 ~ 37.44 ~ 4.80 渭 g / mL-1, respectively. The results of flow curve showed that the separation of liposomes and unencapsulated free drugs by Sephadex LH-20 microcolumn method was effective. The recoveries of free drug DOX and TAM were 98% and 102%, respectively. The results of microcolumn gel separation and HPLC showed that the contents of DOX and TAM in liposomes were about 1 mg mL ~ (-1), and the entrapment efficiency was 90 mg 路mL ~ (-1). Conclusion: this method is simple, rapid, accurate and reliable, and can be used for the determination of DOX and TAM content and encapsulation efficiency in double drug loaded liposomes.
【作者单位】：广州医科大学药学院;广州市第一人民医院放射科;
【基金】：国家自然科学基金资助项目(编号:81402881) 广东省自然科学基金资助项目(编号:S2013040014348) 2014年广东省高校优秀青年培养计划项目(编号:粤教师函【2014】145号) 广州市属高校科研计划项目(编号:2012C210)
【分类号】：R927

【相似文献】