药根碱与DNA G-四链体的相互作用研究
发布时间：2018-07-31 08:59
【摘要】：DNA单链(PS2.M)在K~+存在下可被诱导形成G-四链体。文章利用圆二色谱(CD)以及核磁共振光谱(NMR)表征了G-四链体的形成,并通过荧光滴定光谱、紫外滴定光谱以及核磁共振光谱研究了盐酸药根碱与G-四链体的相互作用。加入G-四链体后,药根碱在530nm处的荧光明显增强;紫外吸收光谱表现出减色和红移现象,说明药根碱可能堆积到G-四链体的尾部或者插入到G-四链体尾部的两个G-四分体之间形成夹心结构。由等摩尔连续变化法算出药根碱和DNA的结合比为1∶1,利用Scatchard方程计算出二者的结合常数为3.12×10~6 L/mol、结合位点为0.98。实验表明:盐酸药根碱与G-四链体有很强的相互作用,药根碱可以稳定DNA的G-四链体结构,二者的作用模式可能为末端堆积。
[Abstract]:DNA single strand (PS2.M) can be induced to form G-4 chain in the presence of K ~. The formation of G- quaternary chain was characterized by circular dichroism (CD) and nuclear magnetic resonance spectroscopy (NMR). The interaction between drug base hydrochloride and G- quaternary was studied by fluorescence titration, ultraviolet titration and nuclear magnetic resonance spectroscopy. After the addition of G-quadruplex, the fluorescence of the root alkaloids at 530nm was obviously enhanced, and the UV absorption spectrum showed the phenomenon of decreasing color and red shift. It is suggested that the nucleotide alkaloids may be stacked at the tail of the G- quadruplex or intercalated into the tail of the G- quaternary to form a sandwich structure between the two G- tetrads. The binding ratio of root alkaloid to DNA is 1: 1, the binding constant is 3.12 脳 10 ~ (6) L / mol and the binding site is 0.98 by Scatchard equation. The results show that there is a strong interaction between the drug root base and the G-quaternary chain, and the drug root alkaloids can stabilize the structure of the G-quaternary chain of DNA. The interaction pattern between them may be terminal stacking.
【作者单位】： 山西大学化学化工学院;山西大学环境科学与工程研究中心;
【基金】：国家自然科学基金(21105060)
【分类号】：R96;O657.3
本文编号：2155083
[Abstract]:DNA single strand (PS2.M) can be induced to form G-4 chain in the presence of K ~. The formation of G- quaternary chain was characterized by circular dichroism (CD) and nuclear magnetic resonance spectroscopy (NMR). The interaction between drug base hydrochloride and G- quaternary was studied by fluorescence titration, ultraviolet titration and nuclear magnetic resonance spectroscopy. After the addition of G-quadruplex, the fluorescence of the root alkaloids at 530nm was obviously enhanced, and the UV absorption spectrum showed the phenomenon of decreasing color and red shift. It is suggested that the nucleotide alkaloids may be stacked at the tail of the G- quadruplex or intercalated into the tail of the G- quaternary to form a sandwich structure between the two G- tetrads. The binding ratio of root alkaloid to DNA is 1: 1, the binding constant is 3.12 脳 10 ~ (6) L / mol and the binding site is 0.98 by Scatchard equation. The results show that there is a strong interaction between the drug root base and the G-quaternary chain, and the drug root alkaloids can stabilize the structure of the G-quaternary chain of DNA. The interaction pattern between them may be terminal stacking.
【作者单位】： 山西大学化学化工学院;山西大学环境科学与工程研究中心;
【基金】：国家自然科学基金(21105060)
【分类号】：R96;O657.3
【相似文献】
相关期刊论文 前2条
1 戈早川,周建明;胶束薄层扫描法测定黄连及其制剂中的小檗碱、巴马汀和药根碱[J];分析化学;2004年01期
2 ;[J];;年期
相关会议论文 前1条
1 曹玉娟;;离子液体-液相色谱法测定中成药中的生物碱含量[A];《分析测试学报》2010年11月增刊2——第四届广东省分析化学研讨会论文集[C];2010年
，本文编号：2155083
本文链接：https://www.wllwen.com/yixuelunwen/yiyaoxuelunwen/2155083.html
最近更新
教材专著
