荧光碳量子点催化鲁米诺发光体系测定2-甲氧基雌二醇
发布时间：2018-08-01 17:20
【摘要】：目的:基于荧光碳量子点(CQDs)的催化特性,建立CQDs-鲁米诺化学发光新体系用于2-甲氧基雌二醇(2-ME)的含量测定。方法:利用微波热解法合成荧光CQDs,采用透射电镜及光谱技术对其进行表征。将CQDs应用于鲁米诺发光体系中,考察不同浓度试剂对发光强度的影响,在最优条件下,对冻干粉剂及血浆样品中的2-ME含量进行测定。结果:CQDs溶液可以增强鲁米诺-铁氰化钾体系的发光信号,因2-ME对这一体系的发光有抑制作用,且该作用与2-ME的浓度呈正比关系,因此建立了检出限为5.4×10-10g/m L,RSD为1.8%(n=11)的2-ME含量测定的新方法。结论:该方法灵敏度高、简单快速,适用于药物制剂及生物样品中2-ME的含量测定。
[Abstract]:Aim: to establish a novel chemiluminescence system for the determination of 2-methoxyestradiol (2-ME) based on the catalytic properties of fluorescent carbon quantum dots (CQDs). Methods: fluorescence CQDs were synthesized by microwave pyrolysis and characterized by transmission electron microscopy and spectroscopy. CQDs was applied to luminol luminescence system. The effects of different concentrations of reagents on luminescence intensity were investigated. Under the optimum conditions, the content of 2-ME in freeze-dried powder and plasma samples was determined. Results the luminescence signal of Lumino-potassium ferricyanide system could be enhanced by the solution of 1: CQDs, because 2-ME inhibited the luminescence of the system, and the effect was proportional to the concentration of 2-ME. Therefore, a new method was established for the determination of 2-ME content with a detection limit of 5.4 脳 10-10g/m L ~ (-1) (n ~ (11). Conclusion: this method is simple and rapid, and is suitable for the determination of 2-ME in pharmaceutical preparations and biological samples.
【作者单位】： 河南省食品药品评价中心;郑州大学药学院药物分析教研室;
【基金】：国家自然科学基金资助项目81503038
【分类号】：O657.3;R927.2
本文编号：2158291
[Abstract]:Aim: to establish a novel chemiluminescence system for the determination of 2-methoxyestradiol (2-ME) based on the catalytic properties of fluorescent carbon quantum dots (CQDs). Methods: fluorescence CQDs were synthesized by microwave pyrolysis and characterized by transmission electron microscopy and spectroscopy. CQDs was applied to luminol luminescence system. The effects of different concentrations of reagents on luminescence intensity were investigated. Under the optimum conditions, the content of 2-ME in freeze-dried powder and plasma samples was determined. Results the luminescence signal of Lumino-potassium ferricyanide system could be enhanced by the solution of 1: CQDs, because 2-ME inhibited the luminescence of the system, and the effect was proportional to the concentration of 2-ME. Therefore, a new method was established for the determination of 2-ME content with a detection limit of 5.4 脳 10-10g/m L ~ (-1) (n ~ (11). Conclusion: this method is simple and rapid, and is suitable for the determination of 2-ME in pharmaceutical preparations and biological samples.
【作者单位】： 河南省食品药品评价中心;郑州大学药学院药物分析教研室;
【基金】：国家自然科学基金资助项目81503038
【分类号】：O657.3;R927.2
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