豚鼠胰腺内telocytes形态学特点及甲磺酸伊马替尼对telocytes的干预作用

发布时间：2018-08-02 14:00

【摘要】：目的对豚鼠胰腺组织特络细胞(telocytes,TCs)的形态特征进行观察与分析,并探讨甲磺酸伊马替尼对TCs的干预作用。方法以健康9周龄豚鼠胰腺为研究对象,利用免疫组化染色对TCs免疫标志(c-kit/CD117、vimentin)进行观察分析。透射电镜观察TCs的超微结构。建立甲磺酸伊马替尼干预模型:豚鼠54只,雌雄不限,分正常对照组、用药组(7、14、21、28 d)、停药组(W7、W14、W21、W28 d);免疫组化染色观察用/停药后TCs变化;Western blot观察用/停药后胰腺组织内CD117、vimentin蛋白表达。结果免疫组化染色显示:胰腺内TCs位于结缔组织内,细胞呈现三角形及长梭形等多种形态,有一至多条长而细的细胞突起,突起包绕腺泡,并相连形成网络结构;电镜超微结构显示:TCs有念珠状的细长突起,核质比较高,胞质内含有少量的线粒体、滑面内质网等;甲磺酸伊马替尼干预后,TCs突起变短,网络结构减少,停药后逐渐恢复;用药后胰腺组织内c-kit/CD117和vimentin蛋白表达降低,停药后逐渐恢复,差异有统计学意义(P0.05)。结论豚鼠胰腺内存在梭形有念珠状突起的TCs,TCs包绕腺泡形成网络结构;甲磺酸伊马替尼可影响胰腺内TCs的形态及蛋白表达。
[Abstract]:Objective to observe and analyze the morphologic characteristics of telocytes-TCs in guinea pig pancreas and to investigate the effect of imatinib mesylate on TCs. Methods Nine-week-old guinea pig pancreas was used to observe and analyze TCs immunological markers (c-kit / CD _ (117) vimentin) by immunohistochemical staining. The ultrastructure of TCs was observed by transmission electron microscope. The model of imatinib mesylate intervention was established: 54 guinea pigs, male and female, were divided into two groups: normal control group and control group. The changes of TCs were observed by immunohistochemical staining of TCs in the treated group (W _ (14) W _ (14) W _ (21) W _ (21) and W _ (28) d); and the expression of CD117vimentin protein in pancreatic tissue was observed by Western blot. Results Immunohistochemical staining showed that TCs in the pancreas was located in connective tissue. The cells were triangular and fusiform. There were one or more long and thin processes around the acinar and connected to form the network structure. The ultrastructure of electron microscope showed that TCs had the slender bulge of rosary, the nucleus and cytoplasm were relatively high, the cytoplasm contained a small amount of mitochondria and the smooth endoplasmic reticulum, etc. After the intervention of imatinib mesylate, the process of TCs became shorter, the network structure decreased, and gradually recovered after drug withdrawal. After treatment, the expression of c-kit/CD117 and vimentin protein in pancreatic tissue decreased, and gradually recovered after withdrawal, the difference was statistically significant (P0.05). Conclusion in the pancreas of guinea pig, the spindle shape and the rosary protuberance form the network structure around the acinar, and imatinib mesylate affects the morphology and protein expression of TCs in the pancreas.
【作者单位】：贵州医科大学基础医学院人体组织学与胚胎学教研室;
【基金】：贵州省科技厅基金{黔科合J字[2008]2277} 贵阳市科技局贵州医科大学联合基金{[20161001]013}~~
【分类号】：R965

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