酮体代谢对大鼠急性脊髓损伤神经保护作用的实验研究

发布时间：2018-08-07 13:31

【摘要】：目的已有研究证实脊髓损伤后的生酮饮食(ketogenic diet,KD)具有神经保护作用,本文进一步探讨损伤前酮体代谢对于大鼠急性颈脊髓挫伤的氧化应激和细胞凋亡的抑制作用。方法 48只雄性Sprague-Dawley大鼠随机分为KD组和标准饮食组(SD),喂养2周后检测血酮体含量。在电磁伺服材料试验机上行C5脊髓挫伤,其挫伤位移为1.5 mm,挫伤速度达500 mm/s。挫伤后3 h和24 h采集颈脊髓组织。采用免疫荧光双标检测与凋亡和炎症相关的Bax,caspase-3和Bcl-2,CD68等的蛋白表达,ELASA检测挫伤部位反映氧化应激程度的SOD和MDA含量。结果 KD组酮体水平为1.32 mmol/L,明显高于SD组(0.38 mmol/L)(P0.05)。Bax,Bcl-2,caspase-3以及CD68的蛋白表达在损伤后均呈阳性,其中KD组表达量在两个时间点上均与SD组的差异有显著性(P0.05)。KD组SOD值在损伤后3h高于SD组,且两组间差异在伤后24 h有显著性(540 pg/ml与281 pg/ml)(P0.05)。MDA的变化趋势与SOD相反,伤后24 h KD组MDA值为509 pg/ml,明显低于SD组(855 pg/ml)(P0.05)。结论本研究结果证明SCI前的高酮体水平减少了急性SCI的氧化应激程度、抑制细胞凋亡,为其在SCI神经保护作用提供了实验证据。
[Abstract]:Objective to investigate the neuroprotective effect of ketogenic diet (ketogenic dietine KD) after spinal cord injury on oxidative stress and apoptosis in acute cervical spinal cord contusion in rats. Methods 48 male Sprague-Dawley rats were randomly divided into KD group and standard diet group. The contusion displacement is 1.5 mm and the contusion speed is 500 mm / s in the ascending C5 spinal cord contusion of the electromagnetic servo material testing machine. Cervical spinal cord tissue was collected 3 and 24 hours after contusion. The expression of Baxia caspase-3 and Bcl-2pCD68 related to apoptosis and inflammation were detected by immunofluorescence double labeling. The contusion sites were detected for the SOD and MDA contents in the contusion area reflecting the degree of oxidative stress. Results the ketone body level in KD group was 1.32 mmol / L, which was significantly higher than that in SD group (0.38 mmol/L) (P0.05). Baxanbcl-2 caspase-3 and CD68 protein expression were positive after injury. The expression of SOD in KD group was significantly higher than that in SD group at two time points (P0.05). The SOD value of KD group was higher than that of SD group at 3 h after injury. There was significant difference between the two groups at 24 h after injury (540 pg/ml vs 281 pg/ml) (P0.05). The change trend of MDA was opposite to that of SOD. The MDA value of KD group was 509pg / ml at 24 h after injury, which was significantly lower than that of SD group (855 pg/ml) (P0.05). Conclusion the results suggest that high ketone body level before SCI can reduce the degree of oxidative stress and inhibit apoptosis of acute SCI, which provides experimental evidence for its neuroprotective effect on SCI.
【作者单位】：南方医科大学南方医院脊柱骨科;
【基金】：国家自然科学基金(81472084)
【分类号】：R965

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