分子排阻色谱-多角度激光散射法测定注射用核糖核酸分子量及其分布
发布时间：2018-08-12 15:00
【摘要】：目的测定注射用核糖核酸中核糖核酸的分子量及其分布。方法采用分子排阻色谱-多角度激光散射联用技术,以含0.02%叠氮钠的0.1 mol·L-1氯化钠溶液为流动相,流速0.5 m L·min-1,采用TSKgel G2000SWXL色谱柱,柱温为30℃。结果获得了样品的dn/dc值、分子量与分子量分布等分子特征参数。测得样品dn/dc值为0.1547,分子量(Mw)在4.6~234.2 k Da,分子量分布系数(Mw/Mn)在1.3~2.9。结论本文尝试以新的思路建立测定生物大分子核糖核酸分子量的方法,该方法快速、简便,可用于核酸类药物分子量及其分布的测定。实际样品检测结果表明,市售的注射用核糖核酸的分子量及分布差异均较大,原料及生产工艺有待进一步规范,加强质量控制。
[Abstract]:Objective to determine the molecular weight and distribution of ribonucleic acid in ribonucleic acid for injection. Methods Molecular exclusion chromatography combined with multi-angle laser scattering was used. The mobile phase was 0.1 mol L-1 sodium chloride solution containing 0.02% sodium azide. The flow rate was 0.5 mL 路min -1. The column temperature was 30 鈩，
本文编号：2179415
[Abstract]:Objective to determine the molecular weight and distribution of ribonucleic acid in ribonucleic acid for injection. Methods Molecular exclusion chromatography combined with multi-angle laser scattering was used. The mobile phase was 0.1 mol L-1 sodium chloride solution containing 0.02% sodium azide. The flow rate was 0.5 mL 路min -1. The column temperature was 30 鈩，
本文编号：2179415
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