青霉素和红霉素对高脂处理小鼠葡萄糖和脂肪代谢的影响

发布时间：2018-08-17 14:14

【摘要】：目的探讨高脂饮食联合低剂量常用抗生素类药物长期暴露对小鼠葡萄糖和脂质代谢的影响。方法将4周龄的C57BL/6J雄性小鼠随机分成5组,每组8只,分别为高脂对照组(Con)、青霉素低浓度组(5 mg/L)(PL)、青霉素高浓度组(50 mg/L)(PH)、红霉素低浓度组(5 mg/L)(EL)、红霉素高浓度组(50 mg/L)(EH)。用高脂饲料联合药物处理90 d,并在75 d进行糖耐受试验。用HE染色法和油红O染色法观察肝脏组织的病理变化。检测血清游离脂肪酸(FFA)、葡萄糖(Glucose)、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDLC),以及肝脏中TG的含量,并采用实时荧光定量PCR技术分析肝脏组织中PPARa和PPARg的基因表达水平的变化。结果抗生素药物处理组的肝脏组织切片显示明显的病理变化,肝脏细胞间脂质堆积明显增加,尤其是EH处理组。与高脂对照组相比,抗生素药物处理组的糖耐量异常,血清FFA、Glucose、TG上升(P0.05),HDL-C呈下降趋势,无显著性。高浓度抗生素药物处理组肝脏中的TG含量和Pparg的表达量都显著高于对照组(P0.05)。结论青霉素和红霉素低剂量长期暴露导致小鼠肝脏中脂质和糖代谢紊乱。
[Abstract]:Objective to investigate the effects of high fat diet combined with low dose antibiotics on glucose and lipid metabolism in mice. Methods four week-old C57BL/6J male mice were randomly divided into 5 groups, 8 in each group, respectively: high fat control group, (Con), penicillin low concentration group (5 mg/L) (PL), penicillin high concentration group (50 mg/L) (PH), erythromycin low concentration group) (5 mg/L) (EL), erythromycin high concentration group (50 mg/L) (EH). High fat diet combined with drugs was used for 90 days and glucose tolerance test was carried out at 75 days. The pathological changes of liver tissue were observed by HE staining and oil red O staining. Serum free fatty acid (FFA), glucose (Glucose), triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) high density lipoprotein cholesterol (HDLC), and liver TG were determined. The changes of gene expression of PPARa and PPARg in liver tissue were analyzed by real-time fluorescence quantitative PCR. Results the histopathological changes and lipid accumulation in liver cells were significantly increased in the antibiotic drug treated group, especially in the EH treated group. Compared with the hyperlipidemia control group, the glucose tolerance was abnormal in the antibiotic drug treatment group, and the serum FFAG glucose TG increased (P0.05) HDL-C showed a downward trend, but no significant difference. The content of TG and the expression of Pparg in liver of high concentration antibiotic treatment group were significantly higher than that of control group (P0.05). Conclusion low dose exposure of penicillin and erythromycin leads to disorder of lipid and glucose metabolism in liver of mice.
【作者单位】：浙江工业大学生物与环境工程学院;
【基金】：国家自然科学基金(21107098) 浙江省新苗人才计划(2014R403027)
【分类号】：R965

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