左卡尼汀对糖尿病心肌病模型大鼠心肌细胞凋亡的影响

发布时间：2018-08-20 14:39

【摘要】：目的:探讨左卡尼汀对糖尿病心肌病模型大鼠心肌细胞凋亡的影响。方法:采用高脂高糖饲料加腹腔注射链脲佐菌素建立糖尿病心肌病大鼠模型,随机分为模型(生理盐水)组和左卡尼汀低、中、高剂量(30、40、50 mg/d)组,另取正常大鼠作为正常对照(纯净水)组,每组10只,ig给予相应药物,每天1次,连续6周。末次给药后检测各组大鼠心室内压、心肌细胞凋亡指数和心肌细胞中B淋巴细胞瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)、p53基因蛋白的表达。结果:与正常对照组和模型组比较,左卡尼汀低、中、高剂量组大鼠的心率、左心室收缩压、左心室内压最大上升和下降速率均降低,左心室舒张末期压升高,差异具有统计学意义(P0.01);与模型组比较,左卡尼汀低、中、高剂量组大鼠的心肌细胞凋亡指数、Bax和p53基因蛋白的表达均明显降低,Bcl-2基因蛋白的表达明显升高,差异具有统计学意义(P0.01或P0.05),其中以左卡尼汀中剂量组效果最显著。结论:左卡尼汀对糖尿病心肌病模型大鼠心肌细胞的凋亡有抑制作用。
[Abstract]:Aim: to investigate the effect of levocarnitine on myocardial apoptosis in diabetic cardiomyopathy rats. Methods: the diabetic cardiomyopathy rats were established by high fat and high glucose diet plus intraperitoneal injection of streptozotocin. The rats were randomly divided into three groups: the model group (normal saline) and the low, medium and high dose group (30 carnitine 50 mg/d). Normal rats were taken as normal control (pure water) group. 10 rats in each group were given corresponding drugs once a day for 6 weeks. After the last administration, intraventricular pressure, myocardial apoptosis index and the expression of B lymphocyte tumor 2 (Bcl-2) Bcl-2 related X protein (Bax) p53 gene were detected in each group. Results: compared with the normal control group and the model group, the heart rate, left ventricular systolic pressure, maximum rise and decrease rate of left ventricular pressure and left ventricular end-diastolic pressure increased in the middle and high dose groups. The difference was statistically significant (P0.01), compared with the model group, the expression of Bcl-2 gene protein and Bax gene protein were significantly decreased in the high dose and middle dose groups. The difference was statistically significant (P0.01 or P0.05), in which L-carnitine medium dose group had the most significant effect. Conclusion: L-carnitine can inhibit cardiomyocyte apoptosis in diabetic cardiomyopathy rats.
【作者单位】：重庆医科大学附属第二医院内分泌科;
【分类号】：R965

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