环氧合酶-1和环氧合酶-2基因多态性与阿司匹林抵抗的相关性研究

发布时间：2018-08-22 07:32

【摘要】：目的探讨环氧合酶-1(COX-1)、环氧合酶-2(COX-2)基因多态性与阿司匹林抵抗相关性。方法将96例缺血性脑卒中患者分为阿司匹林敏感组87例和阿司匹林非敏感组9例。2组患者口服100 mg阿司匹林至少一周。用光比浊法对患者的血小板聚集度进行测定,用Sequenom Mass A RRAY i PLEX基因型分析技术进行COX-1、COX-2的基因型检测,分析COX-1、COX-2基因多态性与阿司匹林的相关性。结果阿司匹林非敏感组患者的既往卒中史比例66.7%(6/9)显著高于阿司匹林敏感组21.9%(19/87)(P0.05)。2组患者的COX-1(rs1330344,rs3842788)基因型分布差异有统计学意义(P0.05),但COX-1(rs5788)、COX-2(rs20417)基因型分布差异无统计学意义(P0.05)。COX-1(rs5788,rs1330344,rs3842788)、COX-2(rs20417)等位基因及基因型频率4个单核苷酸基因多态性均符合Hardy-Weinberg平衡(P0.05)。结论 COX-1(rs3842788,rs1330344)GG型与阿司匹林抵抗密切相关。
[Abstract]:Objective to investigate the association between cyclooxygenase-1 (COX-1), cyclooxygenase--2 (COX-2) gene polymorphism and aspirin resistance. Methods 96 patients with ischemic stroke were divided into aspirin sensitive group (n = 87) and aspirin insensitive group (n = 9). The platelet aggregation of patients was measured by light turbidimetry and the genotypes of COX-2 were detected by Sequenom Mass A RRAY i PLEX genotypic analysis. The correlation between COX-2 gene polymorphism and aspirin was analyzed. Results 66.7% (6 / 9) of patients with previous stroke history in non-sensitive aspirin group were significantly higher than those in aspirin sensitive group (21.9%) (19 / 87) (P0.05). The genotype distribution of COX-1 (rs1330344rs3842788) in aspirin insensitive group was significantly higher than that in aspirin sensitive group (P0.05), but the genotype distribution of COX-2 (rs20417) in COX-1 (rs5788) had no significant difference (P0.05). COX-1 (rs5788-rs1330344rs3842788) was significantly different from that in aspirin sensitive group (P0.05). The four single nucleotide polymorphisms of gene and genotype frequency all accord with Hardy-Weinberg equilibrium (P0.05). Conclusion COX-1 (rs3842788) GG is closely related to aspirin resistance.
【作者单位】：武警浙江总队嘉兴医院药械科;
【分类号】：R969

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