西酞普兰联合石杉碱甲对老年抑郁模型大鼠的改善作用

发布时间：2018-08-22 19:09

【摘要】：目的:研究西酞普兰联合石杉碱甲对老年抑郁大鼠的改善作用。方法:将老年大鼠随机分为空白对照组、模型组、石杉碱甲组(0.3 mg/kg)、西酞普兰组(5 mg/kg)及联用组(石杉碱甲0.3 mg/kg~+西酞普兰5 mg/kg),每组10只。除空白对照组外,其余各组大鼠采用慢性不可预知温和应激方式建立抑郁模型,成模后每天ig给予相应药物1次,连续给药2周。通过旷场实验、糖水消耗实验、悬尾实验、强迫游泳实验、Morris水迷宫实验观察各组大鼠的抑郁和学习记忆行为变化。结果:与空白对照组比较,模型组大鼠水平穿格次数和直立次数、糖水偏好率、站台穿越次数、目标象限路程百分比、目标象限时间百分比均明显减少或降低(P0.05或P0.01),悬尾不动时间、游泳不动时间和逃避潜伏期均明显延长(P0.05或P0.01)。与模型组比较,西酞普兰组和联用组大鼠抑郁相关指标均明显改善(P0.05或P0.01),其中联用组效果更好;联用组大鼠学习记忆相关指标均明显改善(P0.05或P0.01),石杉碱甲组和西酞普兰组大鼠仅站台穿越次数明显增加(P0.05或P0.01),其他学习记忆相关指标无明显变化(P0.05)。结论:西酞普兰联合石杉碱甲能明显改善老年抑郁模型大鼠的抑郁行为和学习记忆能力,效果优于单用西酞普兰。
[Abstract]:Objective: to study the effect of citalopram combined with Huperzine A on senile depression rats. Methods: aged rats were randomly divided into control group, model group, Huperzine A group (0.3 mg/kg), citalopram group (5 mg/kg) and combination group (Huperzine A 0.3 mg / kg ~ citalopram 5 mg/kg) with 10 rats in each group. The depression model was established by chronic unpredictable mild stress in other groups except the blank control group. The rats were given the corresponding drug once a day for 2 weeks after the establishment of the model. The depression and learning and memory behavior of rats in each group were observed by open-field experiment, sugar water consumption test, suspending tail test, forced swimming experiment and Morris water maze test. Results: compared with the blank control group, the rats in the model group had horizontal traversing times and upright times, sugar water preference rate, platform crossing times, target quadrant distance percentage. The percentage of target quadrant time was significantly decreased or decreased (P0.05 or P0.01), the tail suspension time, swimming immobility time and escape latency were significantly prolonged (P0.05 or P0.01). Compared with the model group, citalopram group and combined group were significantly improved in depression related indexes (P0.05 or P0.01). The related indexes of learning and memory were significantly improved in the combined group (P0.05 or P0.01), Huperzine A group and citalopram group only increased the number of platform crossing (P0.05 or P0.01), other learning and memory related indicators did not change significantly (P0.05). Conclusion: citalopram combined with Huperzine A can significantly improve the depressive behavior and learning and memory ability of senile depression model rats, and the effect is better than that of citalopram alone.
【作者单位】：濮阳市卫生学校教务处基础教研室;濮阳市卫生学校科研处;濮阳市人民医院药剂科;
【基金】：河南省科技攻关项目(No.152102310019)
【分类号】：R965

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