不同浓度瑞芬太尼对兔心室肌单相动作电位的电生理特性影响

发布时间：2018-08-23 08:09

【摘要】：目的观察不同浓度瑞芬太尼对心室肌单相动作电位的影响。方法制备Langendorff离体兔心脏模型24个,K-H液灌注15 min后,随机分为4组,每组均6只,对照组继续灌注K-H液60 min;实验组分别灌注含12,25,50ng·m L-1瑞芬太尼的K-H液60 min。平衡灌注15 min(T0)、继续灌注15,30,60 min(T1~T3)时,记录心率、0相最大上升速率(Vmax)、单相动作电位振幅(MAPA)及单相动作电位复极50%,90%的时程(MAPD50,MAPD90)。结果与T0和对照组比较,3个浓度瑞芬太尼组在T1~T3时点,心率明显减慢,MAPD50、MAPD90明显延长(P0.05)。与T0和对照组及低浓度组比较,中、高2个浓度实验组在T1~T3时,Vmax明显减慢,同时MAPA明显降低(P0.05)。结论瑞芬太尼无浓度依赖性地减慢心率、延长动作电位时程;中、高2个浓度瑞芬太尼影响除极,可能是其引起心脏传导阻滞的机制之一。
[Abstract]:Objective to observe the effect of remifentanil at different concentrations on monophasic action potential of ventricular muscle. Methods Langendorff isolated rabbit heart models were perfused with 24 K-H solution for 15 min, and were randomly divided into 4 groups, 6 rats in each group, the control group continued to infuse K-H solution for 60 minutes, and the experimental group was perfused with K-H solution containing 1225 50ng / mL remifentanil for 60 minutes. At 15 min (T _ 0) and 15 ~ 30 ~ 60 min (T1~T3), the maximal rising rate of (Vmax), monophasic action potential (MAPA) and the duration of the repolarization of monophasic action potential (Vmax), _ (50) ~ (90) were recorded during the period of 15 min (T _ 0) and 15 ~ (30) ~ 60 min (T1~T3), respectively. Results compared with T0 and control group, the heart rate of three remifentanil groups was significantly decreased at T1~T3 time (P0.05). Compared with T0, control group and low concentration group, the Vmax and MAPA decreased significantly in the middle and high concentration groups at T1~T3 (P0.05). Conclusion remifentanil reduces heart rate and prolongs the duration of action potential in a concentration-independent manner, and the effect of remifentanil on depolarization may be one of the mechanisms of cardiac block induced by remifentanil.
【作者单位】：贵阳医学院麻醉学系;贵阳医学院附属医院麻醉科;
【基金】：贵州省科技厅联合基金资助项目(黔科合LG字[2011]015号)
【分类号】：R965

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