小剂量联麦氧钒对糖尿病大鼠肝脏及机体氧化应激影响的研究
[Abstract]:OBJECTIVE: As a potential drug for diabetes mellitus, BMOV has shown good hypoglycemic activity. The toxicity and side effects of BMOV have not been fully elucidated. The effect of BMOV on diabetic rats in addition to reducing blood sugar needs further study. Oxidative stress is an important risk factor for diabetic complications. The effect of BMOV on diabetic liver and oxidative stress in diabetic rats is worthy of further study. Methods: 60 healthy male Wistar rats weighing 200-220 g were randomly selected and 50 diabetic rats were induced by STZ. Random blood glucose (>16.7 mmol/L) was used as the criterion to judge the success of diabetic rats. D), 0.5 mg BMOV group (0.5 mg/kg.d), 1 mg BMOV group (1 mg/kg.d), positive control group (1.5 mg/kg.d euglycemic hypoglycemia) and diabetes mellitus model group (equal dose of normal saline), the remaining 10 normal control group (equal dose of normal saline) were given gastric perfusion for three weeks. Serum glycosylated protein, triglyceride, cholesterol, low density lipoprotein, high density lipoprotein, superoxide dismutase, glutathione peroxidase, malondialdehyde, glutamic-pyruvic transaminase, glutamic-oxaloacetic transaminase, body length, liver weight and other indicators were observed by HE staining, and NF-kappa B and TN were detected by immunohistochemical staining. Results: (1) BMOV 0.25 mg group, 0.5 mg group, 1 mg group, positive control group and diabetic model group had lower body weight and body length than normal control group (P BMOV 0.25 mg, 0.5 mg, 1 mg group, positive control group and diabetic model group were higher than normal control group (P 0.05). (3) BMOV 0.25 mg, 0.5 mg, 1 mg group, positive control group, diabetic model group and normal control group were higher in serum triglyceride, cholesterol, low density lipoprotein, high density lipoprotein levels. There was no significant difference in the activity of serum superoxide dismutase (SOD) among the 6 groups (P 0.05) (4); the activity of serum glutathione peroxidase (GSH-Px) in BMOV 0.25 mg group and 0.5 mg group was higher than that in diabetic model group (P 0.05), and there was no significant difference between positive control group and normal control group (P 0.05), but the activity of serum GS in BMOV 1 mg group was higher than that in diabetic model group (P 0.05). The level of serum malondialdehyde (MDA) in BMOV 0.25 mg group was lower than that in diabetic model group (P 0.05), BMOV 0.5 mg group, and 1 mg group was higher than that in normal control group (P 0.05). Compared with normal control group, BMOV 0.25 mg, 0.5 mg, 1 mg group, positive control group and diabetic model group had fat vacuoles and glycogen cavities in the liver, BMOV 1 mg group had inflammatory cell invasion, BMOV 0.25 mg, 0.5 mg, 1 mg group, positive control group and diabetic model group. The results of immunohistochemical staining showed that the expression of NF-kappa B in the liver of BMOV 0.25 mg group, 0.5 mg group, 1 mg group, positive control group and diabetic model group was higher than that of normal control group. The expression of TNF-a in BMOV 0.25 mg group, 0.5 mg group, 1 mg group, positive control group and diabetic model group was higher than that of normal control group. The expression of NF-kappa B and TNF-alpha increased with the increase of BMOV dosage. Conclusion: 1. BMOV 1.0 mg/kg/d and below can not effectively reduce blood glucose in diabetic rats, but blood glucose has shown a decreasing trend. BMOV with dosage of 0.25 mg/kg/d could increase the activity of glutathione peroxidase, decrease the level of malondialdehyde, improve the oxidative stress and antioxidant capacity of diabetic rats. BMOV with dosage of 1 mg/kg/d could decrease the activity of glutathione peroxidase and malondialdehyde in diabetic rats. The increase of aldehyde level may lead to the increase of oxidative stress in diabetic rats. 4. With the increase of BMOV dosage, the liver injury in diabetic rats tends to increase.
【学位授予单位】:吉林大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:R965
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