阿魏植物精油对紫色杆菌群体感应的影响
发布时间：2018-08-24 07:29
【摘要】：本研究采用肉汤微量稀释法对阿魏精油的抗菌活性进行了研究,同时使用紫色杆菌作为细菌模型对阿魏精油的群体抑制剂活力进行了评估。结果表明:阿魏可作为与食品有关微生物和群体感应的抑制剂。阿魏精油的最小抑菌浓度(MIC)值为0.25 mg/m L,可以抑制所有被测细菌的生长。对于阪崎肠杆菌/大肠杆菌/鼠伤寒沙门菌/李斯特菌,其最小杀菌浓度(MBC)值分别为0.25、0.50、0.48、0.48 mg/m L。此外,含浓度为6.25和12.5 mg/m L的阿魏精油纸片显著的抑制紫色杆菌(Chromobacterium violaceum)色素的产生,但不影响紫色杆菌的生长。当浓度被提升到25 mg/m L,既抑制色素的产生也抑制细菌的生长。同样地,所有浓度的阿魏精油抑制了紫色杆菌素的产生,即使最低浓度的阿魏精油0.13 mg/m L,也使色素产物减少38%以上;浓度为0.13、0.50和1.0 mg/m L的阿魏精油不影响紫色杆菌的生存能力,只有最高浓度2.0 mg/m L抑制了细菌的生长,在培养后使细菌数量减少1.37 log CFU/m L。这些结果表明阿魏精油作为一种新型的群体感应抑制剂可有效影响紫色杆菌的群体感应机制。
[Abstract]:The antimicrobial activity of essential oil of Ferula ferulae was studied by broth microdilution method, and the activity of population inhibitor of essential oil of Ferula was evaluated by using purple bacillus as a bacterial model. The results showed that Ferula could be used as a food-related microbial and population-sensitive inhibitor. The minimum inhibitory concentration (MIC) of essential oil was 0.25 mg/m / L, which inhibited the growth of all tested bacteria. For Enterobacter sakazakii / Escherichia coli / Salmonella typhimurium / Listeria, the minimum bactericidal concentration (MBC) was 0.250.50,0.48 mg/m / 0.48 mg/m / L, respectively. In addition, the essential oil paper containing 6.25 and 12.5 mg/m / L of ferulic oil significantly inhibited the production of (Chromobacterium violaceum) pigment from purple bacillus, but did not affect the growth of purple bacillus. When the concentration was raised to 25 mg/m L, both the production of pigment and the growth of bacteria were inhibited. Similarly, all concentrations of ferulic essential oil inhibited the production of purple bacterein. Even the lowest concentration of ferulic essential oil (0.13 mg/m / L) reduced the pigment product by more than 38%, while the concentration of 0.130.50 and 1.0 mg/m / L of ferulic essential oil did not affect the viability of purple bacillus. Only the highest concentration of 2.0 mg/m / L inhibited the growth of bacteria and reduced the number of bacteria by 1.37 log CFU/m / L. These results suggest that ferulic essential oil, as a new type of colony sensing inhibitor, can effectively affect the colony sensing mechanism of purple bacillus.
【作者单位】： 福建农林大学食品科学学院;
【基金】：国家自然科学基金资助项目(31101327) 国家青年科学基金项目资助项目(31401597) 福建省自然科学项目基金资助项目(2013J01084)
【分类号】：R915
本文编号：2200064
[Abstract]:The antimicrobial activity of essential oil of Ferula ferulae was studied by broth microdilution method, and the activity of population inhibitor of essential oil of Ferula was evaluated by using purple bacillus as a bacterial model. The results showed that Ferula could be used as a food-related microbial and population-sensitive inhibitor. The minimum inhibitory concentration (MIC) of essential oil was 0.25 mg/m / L, which inhibited the growth of all tested bacteria. For Enterobacter sakazakii / Escherichia coli / Salmonella typhimurium / Listeria, the minimum bactericidal concentration (MBC) was 0.250.50,0.48 mg/m / 0.48 mg/m / L, respectively. In addition, the essential oil paper containing 6.25 and 12.5 mg/m / L of ferulic oil significantly inhibited the production of (Chromobacterium violaceum) pigment from purple bacillus, but did not affect the growth of purple bacillus. When the concentration was raised to 25 mg/m L, both the production of pigment and the growth of bacteria were inhibited. Similarly, all concentrations of ferulic essential oil inhibited the production of purple bacterein. Even the lowest concentration of ferulic essential oil (0.13 mg/m / L) reduced the pigment product by more than 38%, while the concentration of 0.130.50 and 1.0 mg/m / L of ferulic essential oil did not affect the viability of purple bacillus. Only the highest concentration of 2.0 mg/m / L inhibited the growth of bacteria and reduced the number of bacteria by 1.37 log CFU/m / L. These results suggest that ferulic essential oil, as a new type of colony sensing inhibitor, can effectively affect the colony sensing mechanism of purple bacillus.
【作者单位】： 福建农林大学食品科学学院;
【基金】：国家自然科学基金资助项目(31101327) 国家青年科学基金项目资助项目(31401597) 福建省自然科学项目基金资助项目(2013J01084)
【分类号】：R915
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