孟鲁司特对嗜酸细胞胃肠炎幼鼠肠道炎症的抑制作用

发布时间：2018-08-24 14:43

【摘要】：目的建立SD幼鼠嗜酸细胞胃肠炎(EG)模型,探讨孟鲁司特(MK)对EG幼鼠肠道炎症的抑制作用。方法 32只3周龄雌性SD幼鼠随机分为4组:对照组(M0I0组)、对照干预组(M0I1组)、模型组(M1I0组)、模型干预组(M1I1组)。M1I0组和M1I1组幼鼠以卵白蛋白(OVA)为过敏原,AL(OH)3为佐剂,通过腹腔注射致敏联合灌胃激发建立模型。观察大鼠肠黏膜病理改变、嗜酸性粒细胞(EOS)密度、肥大细胞(MC)密度及脱颗粒情况,采用析因设计方法分析各因素的效果。结果 M1I0组幼鼠空肠黏膜炎症损伤明显,M1I1组幼鼠空肠黏膜炎症损伤减轻。M0I0、M0I1、M1I0及M1I1组幼鼠空肠黏膜中EOS密度分别为(8.6±2.2),(8.4±1.9),(26.4±1.8)和(16.4±2.2),MC密度分别为(2.3±0.4),(2.3±0.4),(13.5±0.7)及(4.3±0.6),MC脱颗粒率分别为18.3(16.7,23.3)%,19.1(17.0,22.9)%,76.8(76.0,76.8)%,45.1(43.0,48.6)%;建立OVA-EG模型可显著增加幼鼠空肠黏膜中EOS密度、MC密度及MC脱颗粒率,使用MK干预则可显著降低幼鼠空肠黏膜中EOS密度、MC密度及MC脱颗粒率;激发后幼鼠空肠黏膜EOS密度、MC密度及MC脱颗粒率均受造模和使用MK干预两因素影响,造模与使用MK干预间存在交互作用。结论 MK可能通过减少肠黏膜EOS、MC浸润,降低MC脱颗粒率,从而减轻OVA致敏激发所致的EG幼鼠肠道炎症损伤。
[Abstract]:Objective to establish a (EG) model of eosinophilic gastroenteritis in young SD rats and to investigate the inhibitory effect of montelukast (MK) on intestinal inflammation in young EG rats. Methods 32 3-week-old female SD young rats were randomly divided into four groups: control group (M0I0 group), control group (M0I1 group), model group (M1I0 group), model intervention group (M1I1 group). M1I0 group and M1I1 group were treated with ovalbumin (OVA) as allergy AL (OH) 3 as adjuvant. The model was established by intraperitoneal injection of sensitization combined with gastric perfusion. The pathological changes of intestinal mucosa, the density of eosinophilic granulocyte (EOS), the density of mast cell (MC) and the degranulation were observed. 缁撴灉 M1I0缁勫辜榧犵┖鑲犻粡鑶滅値鐥囨崯浼ゆ槑鏄，

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