益赛普对脓毒症大鼠心肌损伤与炎症因子表达的影响

发布时间：2018-08-27 14:53

【摘要】：目的研究早期应用益赛普对脓毒症大鼠心肌损伤及炎症因子表达的影响,并就其对心肌损伤机制的影响进行初步探讨。方法 108只雌性清洁级SD大鼠随机分为假手术组、脓毒症组与治疗组,假手术组行盲肠探查术,脓毒症组和治疗组两组行盲肠结扎穿孔术制作脓毒症模型,治疗组加用益赛普以干预,均在3、9、24、36、48、72 h时相点留取标本,每时相点6只大鼠。ELISA法检测大鼠血清心肌肌钙蛋白(cT nI)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)浓度,Western blot法检测心肌核转录因子-κB p65(NF-κB p65)的表达,数据结果采用析因设计方差分析。结果 1脓毒症组与治疗组血清cT nI、TNF-α、IL-1、NF-κB p65水平均高于假手术组(P0.05),两组浓度均于3 h达峰值,而后逐渐下降。治疗组每个时相点均低于脓毒症组;2血清TNF-α、IL-1浓度均与血清CTnI表达呈显著正相关性。结论脓毒症大鼠心肌早期发生损伤,应用益赛普能够降低血清TNF-α、IL-1的释放及心肌组织NF-κB p65的表达,减少机体炎症反应,起到保护心脏的作用。
[Abstract]:Objective to study the effects of early application of Yisepu on myocardial injury and expression of inflammatory factors in septic rats, and to explore the mechanism of myocardial injury. Methods one hundred and eight female clean grade SD rats were randomly divided into sham operation group, sepsis group and treatment group, sham operation group, cecal exploration group, sepsis group and treatment group by cecal ligation and perforation to make sepsis model. In the treatment group, the patients were treated with iseptide for intervention, and the specimens were collected at the time of 72 hours, Serum cardiac troponin (cT nI), tumor necrosis factor- 伪 (TNF- 伪) and interleukin-1 (IL-1) concentration were detected by Elisa and NF- 魏 B p65 expression in 6 rats at each time point. The results were analyzed by factorial design variance analysis. Results 1the serum levels of cT nI,TNF- 伪 -IL-1- 魏 B p65 in sepsis group and treatment group were higher than those in sham operation group (P0.05). The concentration of NF- 魏 B p65 in both groups reached its peak at 3 h, and then decreased gradually. There was a significant positive correlation between serum TNF- 伪 and IL-1 and serum CTnI expression at each time point in the treatment group compared with that in the sepsis group. Conclusion Myocardial injury occurs early in sepsis rats. The application of Isop can reduce the release of TNF- 伪 -IL-1 and the expression of NF- 魏 B p65 in myocardium, reduce the inflammatory reaction and protect the heart.
【作者单位】：安徽医科大学第一附属医院烧伤科;
【基金】：安徽省卫生厅科研基金(编号:09A067)
【分类号】：R965

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