乌司他丁对矽肺大鼠肺组织病理状态与水通道蛋白1及基质金属蛋白酶表达的影响

发布时间：2018-09-03 08:27

【摘要】：目的:研究乌司他丁对矽肺大鼠肺组织病理状态与水通道蛋白1(AQP-1)及基质金属蛋白酶2(MMP-2)、MMP-9表达的影响。方法:取Wistar大鼠分为正常对照组、模型组和乌司他丁高、中、低剂量组(50×10~4、10×10~4、2×10~4u/kg),每组20只。除正常对照组外,其余各组大鼠采用非暴露式气管内注入法灌注40 mg/mL的SiO_2混悬液1 mL建立矽肺模型;建模给药前1 h ip相应剂量的乌司他丁,连续给药3 d。采用苏木精-伊红染色观察各组大鼠肺组织矽结节病理情况;免疫荧光法检测肺组织中AQP-1的表达;实时荧光定量聚合酶链式反应法检测肺组织中MMP-2、MMP-9 m RNA的表达;Western blot法检测肺组织中MMP-2、MMP-9蛋白的表达。结果:模型组大鼠肺组织可见明显矽结节出现,肺组织结构破坏严重;乌司他丁各剂量组大鼠可见含尘巨噬细胞灶,但肺纤维化程度较模型组明显减轻。与正常对照组比较,其余各组大鼠肺组织中AQP-1表达均降低,MMP-2、MMP-9 m RNA及蛋白表达均增强(P0.05);与模型组比较,乌司他丁各剂量组大鼠肺组织中AQP-1表达均增加,MMP-2、MMP-9 m RNA及蛋白表达均减弱(P0.05),其中高、中剂量组改善效果较低剂量组明显(P0.05)。结论:乌司他丁能缓解矽肺模型大鼠病理状态,可上调肺组织中AQP-1表达和下调MMP-2、MMP-9表达。
[Abstract]:Aim: to study the effects of ulinastatin on the expression of aquaporin-1 (AQP-1) and matrix metalloproteinase-2 (MMP-2) MMP-9 in lung tissue of silicosis rats. Methods: Wistar rats were divided into normal control group, model group, ulinastatin high, middle and low dose group (50 脳 10 ~ (4) 10 脳 10 ~ (4) C _ (2 脳 10~4u/kg) with 20 rats in each group. In addition to the normal control group, the silicosis model was established by intratracheal instillation of SiO_2 suspension for 40 mg/mL for 1 mL, and the corresponding dose of ulinastatin 1 h before the administration of ulinastatin was continuously administered for 3 days. Using hematoxylin-eosin staining to observe the pathological situation of the pulmonary tissue of the rats in each group, the expression of AQP-1 in the lung tissue was detected by immunofluorescence method. The expression of MMP-2,MMP-9 m RNA in lung tissue was detected by real-time fluorescence quantitative polymerase chain reaction and the expression of MMP-2,MMP-9 protein in lung tissue was detected by Western blot. Results: the pulmonary tissue of the model group was obviously silicotic nodule, and the lung structure was damaged seriously, and the dust-containing macrophage foci could be seen in each dose of ulinastatin group, but the degree of pulmonary fibrosis was much less than that in the model group. Compared with the normal control group, the expression of AQP-1 in the lung tissues of the other groups decreased significantly, the expression of MMP-2 MMP-9 m RNA and protein increased (P0.05), and compared with the model group, the expression of MMP-2 MMP-9 m RNA and protein increased significantly in the other groups. The expression of AQP-1 in lung tissue of ulinastatin group increased significantly (P0.05), and the expression of MMP-2 MMP-9 m RNA and protein decreased (P0.05), and the improvement effect of Ulinastatin group was higher than that of low dose group (P0.05). Conclusion: ulinastatin can relieve the pathological state of silicosis rats, and can up-regulate the expression of AQP-1 and down-regulate the expression of MMP-2,MMP-9 in lung tissue.
【作者单位】：新乡医学院第一附属医院药学部;新乡医学院第一附属医院神经内科;
【分类号】：R965

【参考文献】