托芬那酸对二甲基肼诱导大鼠大肠癌的拮抗研究
发布时间：2018-09-11 18:48
【摘要】：目的探索托芬那酸对大鼠大肠癌的预防作用。方法选择Wistar清洁级大鼠作为实验对象,随机数字表法随机分为阴性对照组、二甲基肼(dimethylhydrazine,DMH)诱癌组、托芬那酸(tolfenamic acid,TA)对照组和实验组,每组40只大鼠,阴性对照组给予生理盐水皮下注射;DMH诱癌组给予二甲基肼皮下注射建立大鼠大肠癌模型;托芬那酸对照组单纯给予托芬那酸注射液;实验组分为0.1和0.2 ml/kg托芬那酸组,给予二甲基肼溶液皮下注射的同时分别给予0.1和0.2 ml/kg托芬那酸注射液,腹腔注射,1次/d,连续32周,大鼠分别于12和32周进行解剖,观察大鼠的一般状态以及肿瘤发生情况,并对32周的托芬那酸对照组大鼠的形态以及毒性作用进行评价。结果 12周时,各组大鼠均无肿瘤产生;32周时,DMH诱癌组20只,19只产生肿瘤,发生率为95.0%;0.1 ml/kg托芬那酸组20只大鼠,9只产生肿瘤,发生率为45.0%;0.2 ml/kg托芬那酸组20只大鼠,3只产生肿瘤,发生率为15.0%,与DMH诱癌组相比差异具有统计学意义(P0.05);阴性对照组和托芬那酸对照组所有大鼠在实验32周时均未产生肿瘤。对肿瘤大小进行测量,发现实验组的肿瘤直径为1~7 mm,普遍低于DMH诱癌组。托芬那酸对照组所有大鼠一般状态良好,给药32周未出现死亡,体质量与阴性对照组对比差异无统计学意义,出现2例大鼠腹腔给药30 min后出现身体震颤、尾巴隆起症状,1例大鼠发生十二指肠溃疡,肉眼观察肝脏、肾脏、心脏等重要组织器官,未发现任何增生组织。结论托芬那酸对二甲基肼诱发的大鼠大肠癌具有拮抗作用。
[Abstract]:Objective to explore the preventive effect of topenaric acid on colorectal cancer in rats. Methods Wistar clean grade rats were randomly divided into three groups: negative control group, dimethylhydrazine (dimethylhydrazine,DMH) induced cancer group, Topenaric acid (tolfenamic acid,TA) control group and experimental group, with 40 rats in each group. The negative control group was given normal saline subcutaneous injection of DMH-induced carcinoma group to establish the rat model of colorectal cancer by subcutaneous injection of dimethylhydrazine; the tofenac control group was given only topenaric acid injection; the experimental group was divided into 0. 1 and 0. 2 ml/kg tofenac groups. Dimethylhydrazine solution was injected subcutaneously with 0. 1 and 0. 2 ml/kg topenic acid injection respectively. The rats were dissected at 12 and 32 weeks to observe the general state and tumorigenesis of the rats. The morphologic and toxic effects of tofenac control group at 32 weeks were evaluated. Results at the 12th week, no tumor was produced in all groups. At 32 weeks, there were 19 carcinomas in the DMH-induced carcinomas group (n = 19). The incidence of tumor was 95.0 ml/kg / 0. 1 ml/kg topenaric acid group (20 rats) and 9 rats (n = 9). The incidence rate was 45. 0 ml/kg / 0. 2 ml/kg topenic acid group (20 rats) and 3 rats (n = 3). The incidence rate was 15.0 and the difference was statistically significant compared with DMH induced cancer group (P0.05). All the rats in the negative control group and topenaric acid control group did not produce tumor at 32 weeks of experiment. It was found that the tumor diameter of the experimental group was 1 ~ 7 mm, which was lower than that of the DMH induced cancer group. All the rats in the topenaric acid control group were generally in good condition, and no death occurred in 32 weeks after administration, and there was no significant difference in body mass between the two groups. There were 2 rats with tremor after 30 min of intraperitoneal administration. Duodenal ulcer was found in one rat with symptoms of tail protuberance. The liver, kidney, heart and other important tissues and organs were observed with the naked eye. No proliferative tissue was found. Conclusion Tofenaric acid has an antagonistic effect on rat colorectal cancer induced by dimethylhydrazine.
【作者单位】： 四川省三台县人民医院;泰州海达医药科技研究所;
【分类号】：R965
[Abstract]:Objective to explore the preventive effect of topenaric acid on colorectal cancer in rats. Methods Wistar clean grade rats were randomly divided into three groups: negative control group, dimethylhydrazine (dimethylhydrazine,DMH) induced cancer group, Topenaric acid (tolfenamic acid,TA) control group and experimental group, with 40 rats in each group. The negative control group was given normal saline subcutaneous injection of DMH-induced carcinoma group to establish the rat model of colorectal cancer by subcutaneous injection of dimethylhydrazine; the tofenac control group was given only topenaric acid injection; the experimental group was divided into 0. 1 and 0. 2 ml/kg tofenac groups. Dimethylhydrazine solution was injected subcutaneously with 0. 1 and 0. 2 ml/kg topenic acid injection respectively. The rats were dissected at 12 and 32 weeks to observe the general state and tumorigenesis of the rats. The morphologic and toxic effects of tofenac control group at 32 weeks were evaluated. Results at the 12th week, no tumor was produced in all groups. At 32 weeks, there were 19 carcinomas in the DMH-induced carcinomas group (n = 19). The incidence of tumor was 95.0 ml/kg / 0. 1 ml/kg topenaric acid group (20 rats) and 9 rats (n = 9). The incidence rate was 45. 0 ml/kg / 0. 2 ml/kg topenic acid group (20 rats) and 3 rats (n = 3). The incidence rate was 15.0 and the difference was statistically significant compared with DMH induced cancer group (P0.05). All the rats in the negative control group and topenaric acid control group did not produce tumor at 32 weeks of experiment. It was found that the tumor diameter of the experimental group was 1 ~ 7 mm, which was lower than that of the DMH induced cancer group. All the rats in the topenaric acid control group were generally in good condition, and no death occurred in 32 weeks after administration, and there was no significant difference in body mass between the two groups. There were 2 rats with tremor after 30 min of intraperitoneal administration. Duodenal ulcer was found in one rat with symptoms of tail protuberance. The liver, kidney, heart and other important tissues and organs were observed with the naked eye. No proliferative tissue was found. Conclusion Tofenaric acid has an antagonistic effect on rat colorectal cancer induced by dimethylhydrazine.
【作者单位】： 四川省三台县人民医院;泰州海达医药科技研究所;
【分类号】：R965
【相似文献】
相关期刊论文 前3条
1 孙培锋;卢小逸;张启明;;顶空-气相色谱法测定托芬那酸中有机溶剂残留[J];中国新技术新产品;2014年12期
2 范莹;苏R，
本文编号：2237563
本文链接：https://www.wllwen.com/yixuelunwen/yiyaoxuelunwen/2237563.html
最近更新
教材专著
