二甲基甲酰胺对HL7702肝细胞毒性及线粒体膜电位的影响

发布时间：2018-09-13 07:49

【摘要】：目的观察二甲基甲酰胺(DMF)对HL7702肝细胞的细胞活力、凋亡及线粒体膜电位的影响,探讨DMF引起肝细胞凋亡的可能机制。方法 HL7702肝细胞给予50、100、150、200和250 mmol/L DMF处理,于24 h后检测其细胞存活率,乳酸脱氢酶(LDH)释放量。根据细胞毒性测试结果设置对照组及DMF染毒组(200 mmol/L)。流式细胞术检测DMF对HL7702细胞凋亡以及对线粒体膜电位的影响。结果 DMF剂量依赖性地降低HL7702细胞的存活率,其中150、200和250 mmol/L DMF组与对照组相比,细胞存活率明显降低;并且,随着DMF染毒浓度增加,释放至细胞外的LDH的含量增加,与DMF浓度呈正相关。200 mmol/L DMF染毒24 h后,与对照组相比,细胞凋亡率增加,差异有统计学意义(P0.001),线粒体膜电位降低,差异也有统计学意义(P0.001)。结论 DMF对HL7702细胞的毒性作用具有剂量依赖性,DMF引起HL7702线粒体膜电位的改变与DMF导致细胞凋亡有关。
[Abstract]:Objective to observe the effects of dimethylformamide (DMF) on the viability, apoptosis and mitochondrial membrane potential of HL7702 hepatocytes, and to explore the possible mechanism of apoptosis induced by DMF. Methods HL7702 hepatocytes were treated with 50100150200 and 250 mmol/L DMF for 24 h. The survival rate and (LDH) release of lactate dehydrogenase were measured. According to the results of cytotoxicity test, control group and DMF group (200 mmol/L) were set up. The effects of DMF on apoptosis of HL7702 cells and mitochondrial membrane potential were detected by flow cytometry. Results DMF decreased the survival rate of HL7702 cells in a dose-dependent manner. The survival rate of HL7702 cells in the 150200 and 250 mmol/L DMF groups was significantly lower than that in the control group, and the content of LDH released to the extracellular cells increased with the increase of the concentration of DMF. Compared with the control group, the apoptosis rate increased significantly (P0.001), the mitochondrial membrane potential decreased and the difference was statistically significant (P0.001). Conclusion the toxic effect of DMF on HL7702 cells is dose-dependent. The change of mitochondrial membrane potential induced by DMF is related to the apoptosis of HL7702 cells induced by DMF.
【作者单位】：中国疾病预防控制中心职业卫生与中毒控制所;
【基金】：国家自然科学基金(81372963)
【分类号】：R99

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