雷公藤内生真菌Pestalotiopsis disseminata次级代谢产物研究

发布时间：2018-10-09 19:12

【摘要】：采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱及PHPLC等手段对雷公藤(Tripterygium wilfordii Hook.f.)内生真菌广布拟盘多毛孢(Pestalotiopsis disseminata)的发酵次级代谢产物进行分离纯化,通过理化性质及波谱学数据分析对化合物结构进行鉴定。结果表明,从内生真菌Pestalotiopsis disseminata的次级代谢产物中共分离并鉴定了7个单体化合物,分别为7-羟基-5-甲氧基-4,6-二甲基苯酞(Ⅰ)、腺苷(Ⅱ)、尿苷(Ⅲ)、邻苯二甲酸二正丁酯(Ⅳ)、邻苯二甲酸二异丁酯(Ⅴ)、1,1′-二(1,1′-羧基乙基)醚(Ⅵ)、赤藓醇(Ⅶ),其中化合物Ⅱ、Ⅲ、Ⅵ、Ⅶ为首次从该属真菌中分离得到,化合物Ⅰ、Ⅴ为首次从该种真菌中分离得到。
[Abstract]:Determination of Tripterygium wilfordii (Tripterygium wilfordii Hook.f. by silica gel column chromatography and Sephadex LH-20 gel column chromatography and PHPLC The secondary metabolites of endophytic fungus (Pestalotiopsis disseminata) were isolated and purified. The structures of the compounds were identified by physical and chemical properties and spectral data. The results showed that seven monomers were isolated and identified from the secondary metabolites of endophytic fungi Pestalotiopsis disseminata. They are 7-hydroxy-5-methoxy-6-dimethylphthalide (鈪，

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