维生素D及其受体对RIP1调控作用的研究

发布时间：2018-10-17 12:03

【摘要】：目的探讨维生素D及其受体对受体相互作用蛋白(RIP)1表达的影响及其作用机制。方法采用实时定量PCR和Western blot技术,检测维生素D刺激对RIP1的m RNA和蛋白表达水平的影响,采用免疫共沉淀的方法探讨维生素D及其受体对RIP1的调节机制。结果维生素D及其受体能够显著降低RIP1的m RNA及蛋白水平表达,抑制HSP90与RIP1复合物的形成。结论维生素D及其受体通过调节HSP90与RIP1的相互作用调控RIP1的表达,为维生素D及其受体抑制肿瘤的发生提供了新的理论依据。
[Abstract]:Objective to investigate the effect of vitamin D and its receptor on the expression of receptor interacting protein (RIP) 1 and its mechanism. Methods the effects of vitamin D stimulation on the expression of m RNA and protein in RIP1 were detected by real-time quantitative PCR and Western blot. The regulation mechanism of vitamin D and its receptors on RIP1 was investigated by immunoprecipitation. Results Vitamin D and its receptor could significantly reduce the expression of m RNA and protein in RIP1 and inhibit the formation of HSP90 / RIP1 complex. Conclusion Vitamin D and its receptors regulate the expression of RIP1 by regulating the interaction between HSP90 and RIP1, which provides a new theoretical basis for the inhibition of tumor development by vitamin D and its receptors.
【作者单位】： 中国医科大学代谢病分子机制与药物研究所;
【基金】：国家自然科学基金(81270098;81371759)
【分类号】：R96

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