聚乙烯醇-卟啉新型纳米颗粒的制备及其光热特性

发布时间：2018-10-20 07:07

【摘要】：目的将一定比例的脱镁叶绿酸引入聚乙烯醇-硫辛酸聚合物中,分析其理化性质及光热特性。方法采用"一步法"合成聚乙烯醇-硫辛酸-脱镁叶绿酸(PLP)聚合物,激光粒径测定仪测定PLP纳米颗粒(PLPN)的粒径、多分散系数和ζ电位,电镜下观察形态,分光光度法和热成像仪初步探索其体外荧光和光热特性;建立卵巢癌裸鼠模型,行初步的体内实验评价。结果合成的PLP聚合物在水相中自组装形成规则的球状纳米颗粒,电镜下呈紧密的球状结构,平均粒径、多分散系数和ζ电位分别为(22.92±9.00)nm、0.117±0.056和(-25.43±0.77)mV;PLPN在磷酸盐缓冲液(PBS)中的荧光强度明显低于十二烷基硫酸钠(SDS)溶液(P0.05),而近红外光照后温度明显高于SDS溶液(P0.05);在荷瘤裸鼠模型中,PLPN可靶向聚集在肿瘤部位,近红外光激发后能明显提高肿瘤部位的温度,并能杀伤肿瘤细胞。结论 PLPN发射红色荧光,能靶向聚集在肿瘤部位并行活体显像,且在体内外均具有显著的光热转换特性,在体内能有效抑制肿瘤的生长。
[Abstract]:Aim to introduce a certain proportion of demagnesium chloric acid into polyvinyl alcohol-lipoic acid polymer and analyze its physical and chemical properties and photothermal properties. Methods (PLP) polymer was synthesized by "one step method". The particle size, polydispersity coefficient and 味 potential of PLP nanoparticles were measured by laser particle size analyzer, and morphology was observed under electron microscope. The fluorescence and photothermal characteristics of ovarian cancer in vitro were investigated by spectrophotometry and thermal imager, and a nude mouse model of ovarian cancer was established and evaluated in vivo. Results the synthesized PLP polymers were self-assembled in aqueous phase to form regular spherical nanoparticles with compact spherical structure and average particle size under electron microscope. The fluorescence intensity of polydispersity coefficient and 味 potential were (22.92 卤9.00) nm,0.117 卤0.056 and (-25.43 卤0.77) mV;PLPN in phosphate buffer (PBS) significantly lower than those in dodecyl sodium sulfate (SDS) solution (P0.05), but the temperature after near-infrared illumination was significantly higher than that of SDS solution (P0.05). Can be targeted at the tumor site, Near-infrared irradiation can significantly increase the temperature of tumor site and kill tumor cells. Conclusion PLPN emits red fluorescence, can focus on tumor site and imaging in vivo, and has obvious photothermal conversion characteristics in vivo and in vitro, and can effectively inhibit the growth of tumor in vivo.
【作者单位】：解放军陆军总医院生殖中心;解放军第153中心医院普外科;解放军第153中心医院肿瘤科;解放军第155中心医院血液肿瘤科;
【基金】：总后卫生部青年培育项目(14QNP030)
【分类号】：R943

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