CCK-8法检测药物影响肿瘤细胞增殖的优化研究

发布时间：2018-10-22 15:50

【摘要】：目的通过优化CCK-8的实验条件,提高检测药物影响肿瘤细胞增殖的敏感性和可靠性。方法以人肺癌A549细胞和白血病Molt-4细胞为研究对象,CCK-8法和MTT法分别检测细胞增殖的变化,并与Coulter细胞计数法和克隆形成率法进行比较。结果在相同的细胞数下,CCK-8法的检测灵敏度明显高于MTT法。以细胞密度1 000个/孔接种,抗肿瘤药物多柔比星处理96 h后,CCK-8法检测到的变化与克隆形成率法相当。分析Molt-4悬浮细胞发现:CCK-8法与Coulter细胞计数法的检测灵敏度无明显差异(P0.05)。结论优化实验条件后,CCK-8法是一种灵敏度高、可靠性良好的细胞增殖检测方法。
[Abstract]:Objective to improve the sensitivity and reliability of drug detection for tumor cell proliferation by optimizing the experimental conditions of CCK-8. Methods the proliferation of human lung cancer A549 cells and leukemia Molt-4 cells were detected by CCK-8 and MTT, and compared with Coulter cell count and clone formation rate. Results at the same number of cells, the sensitivity of CCK-8 assay was significantly higher than that of MTT method. The cell density was 1 000 / well and the antitumor drug doxorubicin was treated for 96 h. The changes detected by CCK-8 method were similar to those detected by Clone formation method. Analysis of Molt-4 suspension cells showed that there was no significant difference in sensitivity between CCK-8 method and Coulter cell count method (P0.05). Conclusion after optimizing the experimental conditions, CCK-8 method is a sensitive and reliable method for cell proliferation detection.
【作者单位】：中国医学科学院北京协和医学院医药生物技术研究所;
【基金】：国家自然科学基金课题(No.81273553) 北京协和医学院2015年研究生创新基金(No.2015-1007-18)
【分类号】：R96

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