叶绿素非离子表面活性剂囊泡的制备及其理化性质的表征

发布时间：2018-10-23 15:46

【摘要】：目的:制备叶绿素非离子表面活性剂囊泡(chlorophyll nonionic surfactant vesicles,Chl-NS),并对其理化性质进行表征。方法:以非离子表面活性剂脂肪酸山梨坦-80(Span-80)和聚山梨酯-80(Tween-80)复配并结合胆固醇为膜骨架,采用薄膜超声分散法制备叶绿素非离子表面活性剂囊泡;利用透射电镜和Malvern粒度仪分别考察其形态、粒径、分散系数、Zeta电位及稳定性;并进一步考察囊泡对叶绿素的包封率以及包载于囊泡中的叶绿素荧光强度的变化。结果:叶绿素非离子表面活性剂囊泡呈核壳层球形结构,大小均匀,平均粒径为(130.2±10.0)nm,多分散指数(polydispersity index,PDI)值为(0.240±0.01),Zeta电位为(-30.0±5.0)m V,对叶绿素包封率达到(45.2±2.1)%;叶绿素包载于囊泡后,体系的荧光强度明显增强且具有较高的稳定性。结论:本实验制备了稳定性高、粒径均匀、对叶绿素具有良好的包封作用,具有较强近红外荧光特征的非离子表面活性剂囊泡,该囊泡体系有望应用于体内成像系统的相关研究,具有重要临床意义。
[Abstract]:Aim: to prepare chlorophyll-Nonionic surfactant vesicles (chlorophyll nonionic surfactant vesicles,Chl-NS) and characterize its physicochemical properties. Methods: the surfactant vesicles of chlorophyll Nonionic surfactants were prepared by membrane ultrasonic dispersion with the combination of Nonionic surfactant fatty acid sorbitan-80 (Span-80) and polysorbate 80 (Tween-80) and cholesterol as the membrane skeleton. The morphology, particle size, dispersion coefficient, Zeta potential and stability of the vesicles were investigated by TEM and Malvern particle size analysis respectively, and the changes of the encapsulation efficiency of chlorophyll and the fluorescence intensity of chlorophyll embedded in the vesicles were further investigated. Results: chlorophyll Nonionic surfactant vesicles were core-shell spherical and uniform in size. The average diameter of the vesicles was (130.2 卤10.0) nm, polydispersity index (polydispersity index,PDI) was (0.240 卤0.0l), Zeta potential was (-30.0 卤5.0) m V, the encapsulation efficiency of chlorophyll was (45.2 卤2.1)%, and the chlorophyll encapsulation rate was (45.2 卤2.1)%. The fluorescence intensity of the system is obviously enhanced and the system has high stability. Conclusion: Nonionic surfactant vesicles with high stability, uniform particle size, good encapsulation to chlorophyll and strong near infrared fluorescence characteristics were prepared in this experiment. The vesicle system is expected to be used in the study of imaging system in vivo, which has important clinical significance.
【作者单位】：济南大学山东省医学科学院医学与生命科学学院;济宁医学院药学院;
【分类号】：R943

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