结合活体荧光成像技术为两亲性嵌段共聚物建立一种荧光标记方法

发布时间：2018-10-24 15:15

【摘要】：结合活体荧光成像技术,为基于β-环糊精的两亲性嵌段4臂聚合物(β-CD-[P(AA-co-MMA)-b-PVP]4)建立一种荧光标记方法。通过酯交换反应为罗丹明B分子中引入含有双键的甲基丙烯酸羟乙酯,然后通过双键聚合反应将罗丹明B标记到两亲性嵌段共聚物上。借助红外、荧光光谱对标记产物进行表征,并通过紫外分光光度法计算荧光标记产率;制备长春西汀(VP)荧光载药胶束,进行动物组织分布实验和小动物活体荧光成像实验,对比两个实验结果来验证荧光标记结果。荧光标记产率为4.13%;荧光标记前后该聚合物的临界胶束浓度(CMC)基本未改变,分别为5.09×10~(-3)和4.96×10~(-3) mg·L~(-1);对比组织分布和小动物活体荧光成像实验结果发现,二者反映的该胶束在小动物体内的分布情况基本相似,不仅证明了该标记产物的荧光示踪作用,同时结合红外、荧光表征也确证了此荧光标记实验的成功。结合活体荧光成像技术为两亲性嵌段共聚物建立一种荧光标记方法。
[Abstract]:A fluorescence labeling method based on 尾 -cyclodextrin (尾 -cyclodextrin) based amphiphilic block four-arm polymer (尾-CD- [P (AA-co-MMA)-b-PVP] 4) was developed. Hydroxyethyl methacrylate containing double bond was introduced into Rhodamine B molecule by transesterification and then Rhodamine B was labeled onto amphiphilic block copolymer by double bond polymerization. The labeled products were characterized by infrared and fluorescence spectra, and the fluorescence labeling yield was calculated by ultraviolet spectrophotometry. The micelle of vinpocetine (VP) was prepared for animal tissue distribution and in vivo fluorescence imaging of small animals. Two experimental results were compared to verify the fluorescent labeling results. The fluorescence labeling yield was 4.13. The critical micelle concentration (CMC) of the polymer remained unchanged at 5.09 脳 10 ~ (-3) and 4.96 脳 10 ~ (-3) mg L ~ (-1), respectively. The distribution of the micelle in small animals was similar, which not only proved the fluorescence tracer effect of the labeled product, but also confirmed the success of the fluorescence labeling experiment by IR and fluorescence characterization. A fluorescence labeling method for amphiphilic block copolymers was developed with in vivo fluorescence imaging.
【作者单位】：广东药科大学药学院;广东药科大学医药化工学院;
【基金】：广州市科技局科技重大专项资助项目(201300000046) 广东省科技计划资助项目(2013B021800091) 广州市科技计划资助项目(201508010036) 广东药学院科技处-附属医院联合自然培育基金资助项目(GYFYLH201310)
【分类号】：R943

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