二氢杨梅素对湿性年龄相关性黄斑变性的影响

发布时间：2018-10-29 09:10

【摘要】：目的:探讨二氢杨梅素(DMY)对湿性年龄相关性黄斑变性(AMD)的影响。方法:用532 nm激光对成年日本大耳白兔眼底进行光凝,创建AMD模型。光凝4周后,DMY(100 mg·kg~(-1)·d~(-1))灌胃给药,酶联免疫吸附法(ELISA)检测外周血中血管内皮生长因子(VEGF)和色素上皮衍生因子(PEDF)水平;实验结束时,苏木精-伊红染色法检测脉络膜新生血管(CNV);解剖实验动物,分别计算肝脏、肾脏和脾脏指数。结果:给药4周,模型组外周血VEGF降低幅度为63.3%,而模型对照组降低幅度为48.6%;给药8周,模型组外周血VEGF降低幅度为46.7%,模型对照组降低幅度为20.1%。给药4周,模型组外周血PEDF降低幅度为55.9%,而模型对照组降低幅度为58.6%;给药8周,模型组外周血PEDF降低幅度为12.1%,模型对照组降低幅度为53.1%。给药8周,模型对照组可见明显CNV,而模型给药组未见明显CNV;模型给药组肝脏指数和脾脏指数明显低于模型对照组(P=0.147和P=0.223),模型给药组肾脏指数与模型对照组没有明显差异(P=0.874)。结论:DMY具有一定的抑制湿性AMD模型日本大耳白兔CNV生长的药理作用,其作用机制可能与其下调VEGF的表达、上调PEDF表达和益气活血、健脾疏肝有关。
[Abstract]:Objective: to investigate the effect of dihydromyricetin (DMY) on (AMD) of wet age related macular degeneration. Methods: the AMD model was established by photocoagulation on the fundus of adult Japanese white rabbits with 532 nm laser. After 4 weeks of photocoagulation, DMY (100 mg kg~ (-1) d ~ (-1) was administered intragastrically. The levels of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) in peripheral blood were detected by enzyme-linked immunosorbent assay (ELISA). At the end of the experiment, hematoxylin-eosin staining was used to detect the choroidal neovascularization (CNV); in experimental animals, and the indexes of liver, kidney and spleen were calculated. Results: after 4 weeks of administration, the level of VEGF in peripheral blood of model group was 63.3%, while that of model control group was 48.6%. After 8 weeks of administration, the decrease of VEGF in peripheral blood of model group was 46.7%, and that of model control group was 20.1%. After 4 weeks of administration, the decrease of PEDF in peripheral blood of model group was 55.9, that of model control group was 58.6, and that of peripheral blood PEDF was 12.1 in model group and 53.1 in model control group after 8 weeks of administration. After 8 weeks of administration, significant CNV, was found in the model control group, but no significant CNV; was found in the model administration group. The liver index and spleen index in the model group were significantly lower than those in the model control group (P < 0. 147 and P < 0. 223), but there was no significant difference between the model group and the model control group (P < 0. 874). Conclusion: DMY has a certain pharmacological effect on inhibiting the growth of CNV in Japanese large ear white rabbits with wet AMD model, and its mechanism may be related to its down-regulation of VEGF expression, up-regulation of PEDF expression, invigorating qi and activating blood circulation, and invigorating spleen and soothing liver.
【作者单位】：湖北民族学院医学院;湖北民族学院附属民大医院眼科;湖北民族学院生物科学与技术学院;
【基金】：湖北省卫生计生科研基金资助项目(编号:WJ2015MB197)
【分类号】：R965

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